Membrane Association of Presynaptic Cytomatrix Protein Bassoon

• Published in: Biochemical and biophysical research communications 2000-08, Vol.275 (1), p.43-46
• Main Authors: Sanmartí-Vila, Lydia, tom Dieck, Susanne, Richter, Karin, Altrock, Wilko, Zhang, Lixia, Volknandt, Walter, Zimmermann, Herbert, Garner, Craig C., Gundelfinger, Eckart D., Dresbach, Thomas
• Format: Article
• Language: English
• Subjects: active zone; Animals; Blotting, Western; Cell Fractionation; Cell Membrane - chemistry; Cell Membrane - metabolism; Cell Membrane - ultrastructure; Centrifugation, Density Gradient; Cytoskeleton - chemistry; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; Hippocampus - cytology; immunogold EM; Membrane Proteins - metabolism; Microscopy, Immunoelectron; Nerve Tissue Proteins - metabolism; neurotransmitter release; Presynaptic Terminals - metabolism; Presynaptic Terminals - ultrastructure; Rats; synapse; Synaptophysin - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2000.3256

Components of the specialized cytomatrix at active zones of presynaptic nerve terminals are thought to be involved in organizing synaptic events such as immobilisation or translocation of synaptic vesicles and assemblingactive zone components. The 420-kDa non-transmembraneprotein Bassoon is a specific componentof the presynaptic cytomatrix that shares features with both cytoskeleton-associated and peripheral-membrane proteins. Using immunogold electron microscopy we show here that synapse associated Bassoon is distributed in a subregion of active zones. Using a biochemical assay we show that a fraction of Bassoon is membrane associated. Electron microscopy performed on the same biochemical fraction further revealed that Bassoon is associated with vesicular structures. Together these data suggest that at least a fraction of Bassoon is associated with a membraneous compartment in neurons.