Hypothalamic growth hormone secretagogue receptor regulates growth hormone secretion, feeding, and adiposity

Growth hormone secretagogues (GHSs) stimulate GH secretion and food intake. GHS receptor (GHS-R) mRNA has been identified mainly in the arcuate nucleus (Arc) and ventromedial nucleus of the hypothalamus and in the pituitary. Ghrelin, an endogenous ligand for GHS-R, has recently been purified from ra...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of clinical investigation 2002-06, Vol.109 (11), p.1429-1436
Main Authors: Shuto, Yujin, Shibasaki, Tamotsu, Otagiri, Asuka, Kuriyama, Hideki, Ohata, Hisayuki, Tamura, Hideki, Kamegai, Jun, Sugihara, Hitoshi, Oikawa, Shinichi, Wakabayashi, Ichiji
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Animals
Animals, Genetically Modified
Blotting, Western
DNA - metabolism
Dose-Response Relationship, Drug
Female
Ghrelin
Growth Hormone - secretion
Hypothalamus - metabolism
Immunohistochemistry
In Situ Hybridization
Ligands
Male
Oligonucleotides, Antisense - metabolism
Oligopeptides - pharmacology
Peptide Hormones
Peptides - physiology
Promoter Regions, Genetic
Rats
Receptors, Cell Surface - metabolism
Receptors, Cell Surface - physiology
Receptors, G-Protein-Coupled
Receptors, Ghrelin
RNA, Messenger - metabolism
Sex Factors
Stomach - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Growth hormone secretagogues (GHSs) stimulate GH secretion and food intake. GHS receptor (GHS-R) mRNA has been identified mainly in the arcuate nucleus (Arc) and ventromedial nucleus of the hypothalamus and in the pituitary. Ghrelin, an endogenous ligand for GHS-R, has recently been purified from rat stomach. Although ghrelin is also expressed in the hypothalamus, the physiological significance of the ghrelin/GHS-R system is still unknown. We have created transgenic (Tg) rats expressing an antisense GHS-R mRNA under the control of the promoter for tyrosine hydroxylase (TH), thus selectively attenuating GHS-R protein expression in the Arc. Tg rats had lower body weight and less adipose tissue than did control rats. Daily food intake was reduced, and the stimulatory effect of GHS treatment on feeding was abolished in Tg rats. GH secretion and plasma insulin-like growth factor-I levels were reduced in female Tg rats. These results suggest that GHS-R in the Arc is involved in the regulation of GH secretion, food intake, and adiposity.
ISSN:0021-9738
DOI:10.1172/jci0213300