Determination of ethylenediamine tetraacetic acid in injection forms by ion-pair chromatography

A high performance liquid chromatographic method was developed for the determination of ethylenediamine tetraacetic acid (EDTA) in injection forms. The method consists of direct extraction of the samples with ethyl acetate; the organic layers were evaporated to dryness and further diluted to a 0.025...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2002-06, Vol.28 (6), p.1073-1079
Main Authors: Pistos, C., Parissi-Poulou, M.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chelating Agents - analysis
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange - methods
Dosage Forms
Edetic Acid - analysis
EDTA
General pharmacology
High performance liquid chromatography
Injection forms
Ion-pair
Medical sciences
Pharmacology. Drug treatments
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Summary:A high performance liquid chromatographic method was developed for the determination of ethylenediamine tetraacetic acid (EDTA) in injection forms. The method consists of direct extraction of the samples with ethyl acetate; the organic layers were evaporated to dryness and further diluted to a 0.025% (w/v) copper nitrate in order to achive the formation of the EDTA–copper solution complex. The chromatographic separation was performed on a C8 Hypersil column. The mobile phase consisted of a mixture of acetonitrile–0.015 M tetrabutylammonium hydroxide (10:90, v/v), (pH* 7.0) pumped at a flow rate of 1.5 ml min −1. The UV detector was operated at 300 nm. Correlation coefficients of the calibration graphs were better than 0.9995, relative standard deviation was less than 2.5%. Detection limit of EDTA was found to be 1.97 μg ml −1.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(02)00049-3