Anatomical and functional recovery by embryonic stem cell-derived neural tissue of a mouse model of brain damage

We have treated undifferentiated mouse embryonic stem (ES) cells with all- trans retinoic acid (RA) to induce differentiation in vitro into neuron-like cells with good cell viability for use as a graft. Furthermore, we asked whether the RA-induced neuron-like cells restored neurological dysfunction....

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Bibliographic Details
Published in:Journal of the neurological sciences 2004-04, Vol.219 (1), p.107-117
Main Authors: Chiba, Shunmei, Ikeda, Ritsuko, Kurokawa, Manae S., Yoshikawa, Hideshi, Takeno, Mitsuhiro, Nagafuchi, Hiroko, Tadokoro, Mamoru, Sekino, Hiroaki, Hashimoto, Takuo, Suzuki, Noboru
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - pharmacology
Biological and medical sciences
Brain Injuries - pathology
Brain Injuries - therapy
Brain Tissue Transplantation
Cell Differentiation - drug effects
Embryonic stem cell
Endothelium, Vascular - cytology
Hemiplegia
Hemiplegia - pathology
Hemiplegia - therapy
Medical sciences
Mice
Mice, Inbred C57BL
Motor Cortex - injuries
Motor Cortex - pathology
Motor function
Movement
Neural cell
Neurology
Neurons - cytology
Neurons - transplantation
Recovery
Recovery of Function
Retinoic acid
Stem Cell Transplantation
Stem Cells - cytology
Stem Cells - drug effects
Transplantation
Tretinoin - pharmacology
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Summary:We have treated undifferentiated mouse embryonic stem (ES) cells with all- trans retinoic acid (RA) to induce differentiation in vitro into neuron-like cells with good cell viability for use as a graft. Furthermore, we asked whether the RA-induced neuron-like cells restored neurological dysfunction. To this end, the cells were transplanted into right hemiplegia model of mice, developed by a cryogenic injury of motor cortex. Motor function of the recipients was gradually improved, whereas little improvement was observed in control mice. The lesion showed clustering of mature and almost mature neuron-like cells in mice transplanted with the RA-treated cells. The grafted cells had synaptic vesicles. This finding may suggest their maturation and synaptic connection in the recipient brain. Even though further study is necessary to elucidate molecular and cellular mechanisms responsible for the functional recovery, we consider that the ES cells may have advantage for use as a donor source in various neurological disorders including motor dysfunction.
ISSN:0022-510X
1878-5883
DOI:10.1016/j.jns.2004.01.006