Effect of pH on cellulase production of Trichoderma reesei RUT C30

Currently, the high market price of cellulases prohibits commercialization of the lignocellulosics-to-fuel ethanol process, which utilizes enzymes for saccharification of cellulose. For this reason research aimed at understanding and improving cellulase production is still a hot topic in cellulase r...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2004, Vol.113 (1-3), p.201-211
Main Authors: Juhasz, T, Szengyel, Z, Szijarto, N, Reczey, K
Format: Article
Language:English
Subjects:
beta-Glucosidase - chemistry
biological production
Biotechnology - methods
Cellulase - chemistry
cellulases
cellulolytic microorganisms
Cellulose - chemistry
Fermentation
Hydrogen-Ion Concentration
Hypocrea jecorina
Temperature
Time Factors
Trichoderma - chemistry
Trichoderma - enzymology
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Summary:Currently, the high market price of cellulases prohibits commercialization of the lignocellulosics-to-fuel ethanol process, which utilizes enzymes for saccharification of cellulose. For this reason research aimed at understanding and improving cellulase production is still a hot topic in cellulase research. Trichoderma reesei RUT C30 is known to be one of the best hyper producing cellulolytic fungi, which makes it an ideal test organism for research. New findings could be adopted for industrial strains in the hope of improving enzyme yields, which in turn may result in lower market price of cellulases, thus making fuel ethanol more cost competitive with fossil fuels. Being one of the factors affecting the growth and cellulase production of T. reesei, the pH of cultivation is of major interest. In the present work, numerous pH-controlling strategies were compared both in shake-flask cultures and in a fermentor. Application of various buffer systems in shake-flask experiments was also tested. Although application of buffers resulted in slightly lower cellulase activity than that obtained in non-buffered medium, beta-glucosidase production was increased greatly.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:113:1-3:201