Prostate apoptosis after doxazosin treatment in the spontaneous hypertensive rat model

OBJECTIVE To validate the spontaneous hypertensive rat (SHR) model for basic research into benign prostate hyperplasia (BPH), and to assess doxazosin‐induced changes in prostatic structure and apoptotic status. MATERIALS AND METHODS Four groups of rats were assessed: group 1, 15 SHRs treated with do...

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Bibliographic Details
Published in:BJU international 2004-02, Vol.93 (3), p.410-414
Main Authors: Lujan, M., Ferruelo, A., Paez, A., Moreno, A., Berenguer, A.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Antihypertensive Agents - therapeutic use
Apoptosis
Biological and medical sciences
Caspases - metabolism
Cohort Studies
doxazosin
Doxazosin - therapeutic use
Genital system. Reproduction
Hypertension - drug therapy
Hypertension - enzymology
Hypertension - pathology
Male
Medical sciences
Pharmacology. Drug treatments
prostate
Prostatic Hyperplasia - enzymology
Prostatic Hyperplasia - pathology
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - metabolism
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Summary:OBJECTIVE To validate the spontaneous hypertensive rat (SHR) model for basic research into benign prostate hyperplasia (BPH), and to assess doxazosin‐induced changes in prostatic structure and apoptotic status. MATERIALS AND METHODS Four groups of rats were assessed: group 1, 15 SHRs treated with doxazosin; group 2, 14 SHRs with unilateral excision of the major pelvic ganglion; group 3, 14 untreated SHRs; and group 4, 16 intact Wistar‐Kyoto (WKY) rats. The doxazosin mesylate (0.03 mg daily) was given compacted in rat food for 3 months. The prostatic ventral lobe (VL) was excised and weighed. Stereological light microscopy, multiplex reverse transcription‐polymerase chain reaction of prostate caspases, and caspase‐3 activity (cellular enzymatic assay) were assessed. RESULTS There was more development of the glandular epithelium (P 
ISSN:1464-4096
1464-410X
DOI:10.1111/j.1464-410X.2003.04627.x