Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level

We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done i...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2004-04, Vol.126 (13), p.4240-4244
Main Authors: Doré, Kim, Dubus, Sébastien, Ho, Hoang-Anh, Lévesque, Isabelle, Brunette, Maryse, Corbeil, Geneviève, Boissinot, Maurice, Boivin, Guy, Bergeron, Michel G, Boudreau, Denis, Leclerc, Mario
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
DNA - analysis
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Nanotechnology
Polymers - chemistry
RNA - analysis
Spectrometry, Fluorescence - methods
Static Electricity
Thiophenes - chemistry
Various methods and equipments
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Summary:We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja038900d