Three-Dimensional Secretion Signals in Chaperone-Effector Complexes of Bacterial Pathogens

The type III secretion system (TTSS) of Gram-negative bacterial pathogens delivers effector proteins required for virulence directly into the cytosol of host cells. Delivery of many effectors depends on association with specific cognate chaperones in the bacterial cytosol. The mechanism of chaperone...

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Bibliographic Details
Published in:Molecular cell 2002-05, Vol.9 (5), p.971-980
Main Authors: Birtalan, Sara C, Phillips, Rebecca M, Ghosh, Partho
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Outer Membrane Proteins - physiology
Bacterial Proteins - physiology
Catalysis
Crystallography, X-Ray
Macromolecular Substances
Models, Molecular
Molecular Chaperones - physiology
Molecular Conformation
Molecular Sequence Data
Protein Conformation
Protein Denaturation
Stereoisomerism
Trans-Activators - physiology
Yersinia
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Summary:The type III secretion system (TTSS) of Gram-negative bacterial pathogens delivers effector proteins required for virulence directly into the cytosol of host cells. Delivery of many effectors depends on association with specific cognate chaperones in the bacterial cytosol. The mechanism of chaperone action is not understood. Here we present biochemical and crystallographic results on the Yersinia SycE-YopE chaperone-effector complex that contradict previous models of chaperone function and demonstrate that chaperone action is isolated to only a small portion of the effector. This, together with evidence for stereochemical conservation between chaperone-effector complexes, which are otherwise unrelated in sequence, indicates that these complexes function as general, three-dimensional TTSS secretion signals and may endow a temporal order to secretion.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(02)00529-4