Taenia solium: identification of specific antibody binding regions of metacestode 10-kDa protein

• Published in: Experimental parasitology 2002-02, Vol.100 (2), p.87-94
• Main Authors: Chung, Joon-Yong, Yun, Doo-Hee, Eom, Keeseon S., Kang, Shin-Yong, Kong, Yoon, Cho, Seung-Yull
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibodies, Helminth - immunology; Antigens, Helminth - chemistry; Antigens, Helminth - immunology; Biological and medical sciences; Cysticercus - immunology; Diseases caused by cestodes; Epitopes - analysis; Experimental helminthic diseases. Models; Helminth Proteins - chemistry; Helminth Proteins - immunology; Helminthic diseases; Humans; Immunoblotting; Infectious diseases; Medical sciences; Molecular Sequence Data; Neurocysticercosis - diagnosis; Neurocysticercosis - immunology; Neurocysticercosis - parasitology; Parasitic diseases; Precipitin Tests; Sequence Homology, Amino Acid; Species Specificity; Swine; Taeniases
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/S0014-4894(02)00002-4

Taenia solium neurocysticercosis (NCC) represents one of the major public health problems associated with several neurological manifestations worldwide. We previously identified a recombinant 10-kDa protein of T. solium metacestode (CyDA) specific to active NCC. Immunoblottings with sera from active NCC patients and from animals experimentally infected with larval T. solium (pig), T. saginata (pig), T. asiatica (pig), and T. crassiceps (mouse) strongly recognized CyDA, while sera from patients infected only with adult worms did not. Mapping of antigenic sites using deletion mutants revealed that amino acids (aa) residues 30–34, Asn-Met-Thr-Val-Met (NMTVM), reacted only with sera from active stage T. solium cysticercosis cases. Recognition of CyDA aa 30–34 resided almost exclusively in the IgG4 isotype. Competitive immunoprecipitation with synthetic peptides confirmed the specificity of anti-sera for this penta-peptide. These results demonstrated that aa residues NMTVM in CyDA comprise the core sequence for an active stage NCC-related antigenic determinant. Index Descriptors and Abbreviations: Taenia solium metacestode; Cestode; Neurocysticercosis; Specific antigen; Epitope; Larval stage; Genus-/species-specificity; Hydrophobic ligand binding protein; Neurocysticercosis, NCC; T. solium metacestode, TsM; 10-kDa protein of TsM, CyDA; Amino acids, aa; Central nervous system, CNS; Hydrophobic ligand binding protein, HLBP; Cyst fluid, CF; Pooled serum of 10 active NCC patients, serum-pool.