Estrogenic and Antiestrogenic Activities of the Roots of Moghania philippinensis and Their Constituents

In the course of our search for natural estrogenic compounds from medicinal plants, we found that the methanolic extract from the roots of Moghania philippinensis (Fabaceae) showed significant effects on the proliferation of MCF-7 cells (human breast cancer) and induction of β-galactosidase activity...

Full description

Saved in:
Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2004, Vol.27(4), pp.548-553
Main Authors: Ahn, Eun-Mi, Nakamura, Norio, Akao, Teruaki, Nishihara, Tsutomu, Hattori, Masao
Format: Article
Language:English
Subjects:
8-(1,1-dimethylallyl)genistein
Animals
Cell Proliferation - drug effects
Estrogen Antagonists - isolation & purification
Estrogen Antagonists - pharmacology
Estrogens - isolation & purification
Estrogens - pharmacology
Fabaceae - chemistry
Female
Genistein - analogs & derivatives
Genistein - chemistry
Genistein - isolation & purification
Genistein - pharmacokinetics
Genistein - pharmacology
Humans
In Vitro Techniques
MCF-7
Moghania philippinensis
Organ Size - drug effects
Ovariectomy
phytoestrogen
Plant Extracts - pharmacology
Plant Roots - chemistry
Rats
Rats, Sprague-Dawley
Spleen - drug effects
Thymus Gland - drug effects
Tumor Cells, Cultured
Two-Hybrid System Techniques
Uterus - drug effects
yeast two-hybrid assay
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the course of our search for natural estrogenic compounds from medicinal plants, we found that the methanolic extract from the roots of Moghania philippinensis (Fabaceae) showed significant effects on the proliferation of MCF-7 cells (human breast cancer) and induction of β-galactosidase activity in a yeast two-hybrid assay. Through estrogenic activity-guided fractionation, we isolated several active flavonoids including prenylated ones. The CHCl3 fraction and its new constituent, 8-(1,1-dimethylallyl)genistein (9), appreciably increased the uterine weight in ovariectomized rats when administered orally for 14 consecutive days, in which compound 9 showed stronger estrogenic activity than genistein. Antiestrogenic activities were also examined based on the inhibition of MCF-7 cell proliferation and β-galactosidase activity in the yeast two-hybrid assay, mediated by 17β-estradiol. 5,7,3′,4′-Tetrahydroxy-6,8-diprenylisoflavone (6) showed the strongest antiestrogenic activity.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.27.548