Glucose oxidase catalysed oxidation of glucose in a dialysis membrane electrochemical reactor (D-MER)

The purpose of this work was to evaluate the effectiveness of a new Membrane Electrochemical Reactor (MER) for the production of gluconic acid by glucose oxidase (GOD) catalysed glucose oxidation. The GOD was confined against the electrode surface with a dialysis membrane. The role of the electroche...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2004-04, Vol.26 (3), p.165-168
Main Authors: Basséguy, R, Délécouls-Servat, K, Bergel, A
Format: Article
Language:English
Subjects:
Aspergillus niger - enzymology
Dialysis
Electrochemistry
Electrodes
Enzyme kinetics
Gluconates - chemistry
Glucose - chemistry
Glucose Oxidase - chemistry
Hemodialysis
Hydrogen peroxide
Hydrogen Peroxide - chemistry
Kinetics
Membranes, Artificial
Oxidation
Oxidation-Reduction
Reactors
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Summary:The purpose of this work was to evaluate the effectiveness of a new Membrane Electrochemical Reactor (MER) for the production of gluconic acid by glucose oxidase (GOD) catalysed glucose oxidation. The GOD was confined against the electrode surface with a dialysis membrane. The role of the electrochemical step was to eliminate by oxidation the hydrogen peroxide that appeared as a by-product of the reaction and strongly inhibited and/or inactivated GOD. The dialysis MER gave a transformation ratio of 30% with an initial glucose concentration of around 300 mM. This result is significantly better than the maximum of 10% obtained when hydrogen peroxide was eliminated by addition of a large excess of catalase in solution, as is generally done. The D-MER also revealed unexpected properties of the enzyme kinetics, such as an oscillatory behaviour, which were discussed.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-004-0351-7