Passive immunization of rams ( Ovis aries) against GnRH: effects on antibody titer, serum concentrations of testosterone, and sexual behavior

The effect of immunoneutralization of gonadotropin-releasing hormone (GnRH) on serum concentrations of testosterone and sexual behavior was evaluated in sexually mature male sheep. In Experiment 1, GnRH1 rams ( n=16) were passively immunized against GnRH (300 ml antiserum), control rams were either...

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Bibliographic Details
Published in:Animal reproduction science 2002-06, Vol.71 (3), p.203-215
Main Authors: Parthasarathy, V, Price, E.O, Orihuela, A, Dally, M.R, Adams, T.E
Format: Article
Language:English
Subjects:
Animals
Anti-GnRH
Antibodies - blood
Female
Gonadotropin-Releasing Hormone - immunology
Gonadotropin-Releasing Hormone - physiology
Immunization, Passive
Kinetics
Male
Orchiectomy - veterinary
Ovariectomy - veterinary
Passive immunization
Rams
Sexual behavior
Sexual Behavior, Animal
Sheep
Sheep - physiology
Testosterone
Testosterone - blood
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Summary:The effect of immunoneutralization of gonadotropin-releasing hormone (GnRH) on serum concentrations of testosterone and sexual behavior was evaluated in sexually mature male sheep. In Experiment 1, GnRH1 rams ( n=16) were passively immunized against GnRH (300 ml antiserum), control rams were either passively immunized against keyhole limpet hemocyanin (KLH, n=15) or surgically castrated (Wethers1, n=4). Sexual performance of the rams was assessed weekly for 3 weeks before and 6 weeks after immunization, using ovarihystertomized ewes actively immunized against GnRH. Experiment 2 evaluated the effects of repeated immunization. Rams were immunized with two aliquots (400 and 300 ml, respectively) of anti-GnRH sera (GnRH, n=5) or normal sheep serum (NSS, n=4), 2 weeks apart. Surgically castrated animals were used as a second control group (Wethers2). Administration of anti-GnRH sera, but neither anti-KLH nor NSS sera, resulted in marked reduction ( P
ISSN:0378-4320
1873-2232
DOI:10.1016/S0378-4320(02)00028-3