Forensic DNA-typing of dog hair: DNA-extraction and PCR amplification

The forensic application of DNA-typing for the identification of dog hair provides objective evidence in the characterisation of traces found at crime scenes. During the past few years forensic dog identity testing has been improved considerably using multiplex PCR systems. However, DNA-typing from...

Full description

Saved in:
Bibliographic Details
Published in:Forensic science international 2004-05, Vol.141 (2-3), p.149-151
Main Authors: Pfeiffer, I., Völkel, I., Täubert, H., Brenig, B.
Format: Article
Language:English
Subjects:
Amplification
Animals
Biological and medical sciences
Buffers
Ca2+-extraction buffer
Calcium
Calcium ions
Classical genetics, quantitative genetics, hybrids
Crime
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - isolation & purification
DNA testing
Dog hair
Dogs
Dogs - genetics
Forensic genetics
Forensic Medicine - methods
Forensic science
Forensic sciences
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
Hair
Hair - chemistry
Human
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Methods
Mitochondrial DNA
Polymerase Chain Reaction
Roots
Sequence Analysis, DNA
Species Specificity
Success
Tandem Repeat Sequences
Typing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The forensic application of DNA-typing for the identification of dog hair provides objective evidence in the characterisation of traces found at crime scenes. During the past few years forensic dog identity testing has been improved considerably using multiplex PCR systems. However, DNA-typing from samples of one up to 10 dog hairs is often problematic in forensic science. A single dog hair contains very small quantities of DNA or the hair sample consists of hairs with roots of bad quality or even of broken hairshafts without roots. Here we describe an experimental study about dog hairs by means of a Ca2+ improved DNA-extraction method, quantification and amplification.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2004.01.016