A Rapid Screening Method to Monitor Expression of Recombinant Proteins from Various Prokaryotic and Eukaryotic Expression Systems Using Matrix-Assisted Laser Desorption Ionization–Time-of-Flight Mass Spectrometry

Rapid methods using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry to monitor recombinant protein expression from various prokaryotic and eukaryotic cell culture systems were devised. Intracellular as well as secreted proteins from both induced and constitutive expressi...

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Bibliographic Details
Published in:Analytical biochemistry 2002-06, Vol.305 (2), p.242-250
Main Authors: Jebanathirajah, Judith A., Andersen, Søren, Blagoev, Blagoy, Roepstorff, Peter
Format: Article
Language:English
Subjects:
Animals
Cell Line - metabolism
Chromatography, Affinity
Escherichia coli
expression
high throughput
Humans
Insecta
mass spectrometry
Pichia
recombinant protein
Recombinant Proteins - analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Rapid methods using matrix-assisted laser desorption ionization–time-of-flight mass spectrometry to monitor recombinant protein expression from various prokaryotic and eukaryotic cell culture systems were devised. Intracellular as well as secreted proteins from both induced and constitutive expression systems were measured and monitored from whole cells and growth media, thus providing an alternative to time-consuming traditional methods for screening and monitoring of protein expression. The methods described here involve minimal processing of samples and are therefore relevant to high-throughput screening applications.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2002.5681