Measuring mitochondrial reactive oxygen species

This article examines recent methods for measuring reactive oxygen species produced in isolated mitochondria and within live cells, with particular emphasis on the detection of hydrogen peroxide. Protocols for reliable measurements of mitochondrial hydrogen peroxide are presented, while the advantag...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2002-04, Vol.26 (4), p.335-340
Main Author: Degli Esposti, Mauro
Format: Article
Language:English
Subjects:
Animals
Biochemistry - methods
Cells, Cultured
Humans
Hydrogen Peroxide - pharmacology
Lipid Peroxidation
Mitochondria - metabolism
Oxidation-Reduction
Reactive Oxygen Species - analysis
Reactive Oxygen Species - metabolism
Superoxides
Time Factors
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Description
Summary:This article examines recent methods for measuring reactive oxygen species produced in isolated mitochondria and within live cells, with particular emphasis on the detection of hydrogen peroxide. Protocols for reliable measurements of mitochondrial hydrogen peroxide are presented, while the advantages and pitfalls of these and other methods are discussed. New developments in the detection of lipid peroxidation are outlined. Advice is also provided to aid the interpretation of cellular data with respect to the contribution of oxygen radical production by different components of the mitochondrial respiratory chain.
ISSN:1046-2023
1095-9130
DOI:10.1016/S1046-2023(02)00039-7