Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration

Abstract A white-rot basidiomycete Ganoderma lucidum has long been used as a medicinal mushroom in Asia, and it has an array of enzymes important for wood degrading activity. There have been many reports about the ingredients which show health aiding effects. In order to analyze gene functions and i...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2004-04, Vol.233 (2), p.201-204
Main Authors: Kim, Sunkyung, Song, Jaemahn, Choi, Hyoung T.
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Basidiocarps
Biological and medical sciences
Chromosomes
Cloning, Molecular
Deoxyribonuclease EcoRI - genetics
Deoxyribonucleases, Type II Site-Specific - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal transformation
Fungi
Ganoderma lucidum
Genes
Genetic Testing - methods
Genetic transformation
Geneticin
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Integration
Microbiology
Miscellaneous
Mushrooms
Mutation
Mycology
Phosphinothricin
Plasmids
Reishi - genetics
Rot
Transformation, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43
cites cdi_FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43
container_end_page 204
container_issue 2
container_start_page 201
container_title FEMS microbiology letters
container_volume 233
creator Kim, Sunkyung
Song, Jaemahn
Choi, Hyoung T.
description Abstract A white-rot basidiomycete Ganoderma lucidum has long been used as a medicinal mushroom in Asia, and it has an array of enzymes important for wood degrading activity. There have been many reports about the ingredients which show health aiding effects. In order to analyze gene functions and introduce foreign genes into this fungus, genetic transformation is required. We have successfully transformed G. lucidum to geneticin resistance using pJS205-1 which has the antibiotic resistance genes against geneticin and phosphinothricin. Many different mutants have been generated during the transformation by restriction enzyme mediated integration, and the transformation yield was 4–17 transformants (μg plasmid DNA)−1. The plasmid was integrated stably into the recipient chromosome, which was confirmed by PCR with the plasmid-specific primers.
doi_str_mv 10.1111/j.1574-6968.2004.tb09483.x
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71800674</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.2004.tb09483.x</oup_id><sourcerecordid>2307167646</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43</originalsourceid><addsrcrecordid>eNqVkc-P1CAcxYnRuOPqv2Aajd5aoVCgHkzMxh1NxnjRM-FXDWMLI9C4418v3TZqjB7kQgKf9-XxHgBPEGxQWS-ODeoYqWlPedNCSJqsYE84bm7ugN3Pq7tgBzHjNYI9uwAPUjrCwraQ3gcXqIMUE8524MveepudrnKUPg0hTjK74CvpTTXNWfpcuRTG9dD5ai99MLZQ1ThrZ-apUucq2pSj07eM9d_Pk60na5zM1hRNtp_jrf4huDfIMdlH234JPl2_-Xj1tj582L-7en2oNW4RqrVWjLacSqkUwVyanltimBowNAPXarCt7IjRDPdU6xZybaXSEENqEes0wZfg-Tr3FMPXuXgTk0vajqP0NsxJMMQhpGwBn_4BHsMcffEmWgwZoowSWqiXK6VjSCnaQZyim2Q8CwTFUog4iiV1saQulkLEVoi4KeLH2xOzKpn8km4NFODZBsik5TiUGrRLv3EUc4Jx4V6t3Dc32vN_WBDX7w8tRGVAtw4I8-kf8vpvP_gBW9K6rw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2307167646</pqid></control><display><type>article</type><title>Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration</title><source>Oxford Journals Online</source><creator>Kim, Sunkyung ; Song, Jaemahn ; Choi, Hyoung T.</creator><creatorcontrib>Kim, Sunkyung ; Song, Jaemahn ; Choi, Hyoung T.</creatorcontrib><description>Abstract A white-rot basidiomycete Ganoderma lucidum has long been used as a medicinal mushroom in Asia, and it has an array of enzymes important for wood degrading activity. There have been many reports about the ingredients which show health aiding effects. In order to analyze gene functions and introduce foreign genes into this fungus, genetic transformation is required. We have successfully transformed G. lucidum to geneticin resistance using pJS205-1 which has the antibiotic resistance genes against geneticin and phosphinothricin. Many different mutants have been generated during the transformation by restriction enzyme mediated integration, and the transformation yield was 4–17 transformants (μg plasmid DNA)−1. The plasmid was integrated stably into the recipient chromosome, which was confirmed by PCR with the plasmid-specific primers.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2004.tb09483.x</identifier><identifier>PMID: 15063487</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antibiotic resistance ; Antibiotics ; Basidiocarps ; Biological and medical sciences ; Chromosomes ; Cloning, Molecular ; Deoxyribonuclease EcoRI - genetics ; Deoxyribonucleases, Type II Site-Specific - genetics ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Fungal transformation ; Fungi ; Ganoderma lucidum ; Genes ; Genetic Testing - methods ; Genetic transformation ; Geneticin ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Integration ; Microbiology ; Miscellaneous ; Mushrooms ; Mutation ; Mycology ; Phosphinothricin ; Plasmids ; Reishi - genetics ; Rot ; Transformation, Genetic</subject><ispartof>FEMS microbiology letters, 2004-04, Vol.233 (2), p.201-204</ispartof><rights>2004 Federation of European Microbiological Societies 2004</rights><rights>2004 INIST-CNRS</rights><rights>2004 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43</citedby><cites>FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15638433$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15063487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sunkyung</creatorcontrib><creatorcontrib>Song, Jaemahn</creatorcontrib><creatorcontrib>Choi, Hyoung T.</creatorcontrib><title>Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract A white-rot basidiomycete Ganoderma lucidum has long been used as a medicinal mushroom in Asia, and it has an array of enzymes important for wood degrading activity. There have been many reports about the ingredients which show health aiding effects. In order to analyze gene functions and introduce foreign genes into this fungus, genetic transformation is required. We have successfully transformed G. lucidum to geneticin resistance using pJS205-1 which has the antibiotic resistance genes against geneticin and phosphinothricin. Many different mutants have been generated during the transformation by restriction enzyme mediated integration, and the transformation yield was 4–17 transformants (μg plasmid DNA)−1. The plasmid was integrated stably into the recipient chromosome, which was confirmed by PCR with the plasmid-specific primers.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Basidiocarps</subject><subject>Biological and medical sciences</subject><subject>Chromosomes</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonuclease EcoRI - genetics</subject><subject>Deoxyribonucleases, Type II Site-Specific - genetics</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal transformation</subject><subject>Fungi</subject><subject>Ganoderma lucidum</subject><subject>Genes</subject><subject>Genetic Testing - methods</subject><subject>Genetic transformation</subject><subject>Geneticin</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Integration</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mushrooms</subject><subject>Mutation</subject><subject>Mycology</subject><subject>Phosphinothricin</subject><subject>Plasmids</subject><subject>Reishi - genetics</subject><subject>Rot</subject><subject>Transformation, Genetic</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqVkc-P1CAcxYnRuOPqv2Aajd5aoVCgHkzMxh1NxnjRM-FXDWMLI9C4418v3TZqjB7kQgKf9-XxHgBPEGxQWS-ODeoYqWlPedNCSJqsYE84bm7ugN3Pq7tgBzHjNYI9uwAPUjrCwraQ3gcXqIMUE8524MveepudrnKUPg0hTjK74CvpTTXNWfpcuRTG9dD5ai99MLZQ1ThrZ-apUucq2pSj07eM9d_Pk60na5zM1hRNtp_jrf4huDfIMdlH234JPl2_-Xj1tj582L-7en2oNW4RqrVWjLacSqkUwVyanltimBowNAPXarCt7IjRDPdU6xZybaXSEENqEes0wZfg-Tr3FMPXuXgTk0vajqP0NsxJMMQhpGwBn_4BHsMcffEmWgwZoowSWqiXK6VjSCnaQZyim2Q8CwTFUog4iiV1saQulkLEVoi4KeLH2xOzKpn8km4NFODZBsik5TiUGrRLv3EUc4Jx4V6t3Dc32vN_WBDX7w8tRGVAtw4I8-kf8vpvP_gBW9K6rw</recordid><startdate>200404</startdate><enddate>200404</enddate><creator>Kim, Sunkyung</creator><creator>Song, Jaemahn</creator><creator>Choi, Hyoung T.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200404</creationdate><title>Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration</title><author>Kim, Sunkyung ; Song, Jaemahn ; Choi, Hyoung T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Basidiocarps</topic><topic>Biological and medical sciences</topic><topic>Chromosomes</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonuclease EcoRI - genetics</topic><topic>Deoxyribonucleases, Type II Site-Specific - genetics</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal transformation</topic><topic>Fungi</topic><topic>Ganoderma lucidum</topic><topic>Genes</topic><topic>Genetic Testing - methods</topic><topic>Genetic transformation</topic><topic>Geneticin</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Integration</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mushrooms</topic><topic>Mutation</topic><topic>Mycology</topic><topic>Phosphinothricin</topic><topic>Plasmids</topic><topic>Reishi - genetics</topic><topic>Rot</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sunkyung</creatorcontrib><creatorcontrib>Song, Jaemahn</creatorcontrib><creatorcontrib>Choi, Hyoung T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sunkyung</au><au>Song, Jaemahn</au><au>Choi, Hyoung T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2004-04</date><risdate>2004</risdate><volume>233</volume><issue>2</issue><spage>201</spage><epage>204</epage><pages>201-204</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract A white-rot basidiomycete Ganoderma lucidum has long been used as a medicinal mushroom in Asia, and it has an array of enzymes important for wood degrading activity. There have been many reports about the ingredients which show health aiding effects. In order to analyze gene functions and introduce foreign genes into this fungus, genetic transformation is required. We have successfully transformed G. lucidum to geneticin resistance using pJS205-1 which has the antibiotic resistance genes against geneticin and phosphinothricin. Many different mutants have been generated during the transformation by restriction enzyme mediated integration, and the transformation yield was 4–17 transformants (μg plasmid DNA)−1. The plasmid was integrated stably into the recipient chromosome, which was confirmed by PCR with the plasmid-specific primers.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>15063487</pmid><doi>10.1111/j.1574-6968.2004.tb09483.x</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 2004-04, Vol.233 (2), p.201-204
issn 0378-1097
1574-6968
language eng
recordid cdi_proquest_miscellaneous_71800674
source Oxford Journals Online
subjects Antibiotic resistance
Antibiotics
Basidiocarps
Biological and medical sciences
Chromosomes
Cloning, Molecular
Deoxyribonuclease EcoRI - genetics
Deoxyribonucleases, Type II Site-Specific - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal transformation
Fungi
Ganoderma lucidum
Genes
Genetic Testing - methods
Genetic transformation
Geneticin
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Integration
Microbiology
Miscellaneous
Mushrooms
Mutation
Mycology
Phosphinothricin
Plasmids
Reishi - genetics
Rot
Transformation, Genetic
title Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzyme-mediated integration
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T08%3A58%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20transformation%20and%20mutant%20isolation%20in%20Ganoderma%20lucidum%20by%20restriction%20enzyme-mediated%20integration&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Kim,%20Sunkyung&rft.date=2004-04&rft.volume=233&rft.issue=2&rft.spage=201&rft.epage=204&rft.pages=201-204&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.2004.tb09483.x&rft_dat=%3Cproquest_cross%3E2307167646%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3211-ccb76286aabb438ad98e4d7bf30df8cbfe2a54dc7396cc208ceabc0306e175c43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2307167646&rft_id=info:pmid/15063487&rft_oup_id=10.1111/j.1574-6968.2004.tb09483.x&rfr_iscdi=true