Identification of proteins differentially expressed during chondrogenesis of mesenchymal cells

• Published in: FEBS letters 2004-04, Vol.563 (1), p.35-40
• Main Authors: Lee, Sun Joo, Jeon, Hong Bae, Lee, Jeung Hwa, Yoo, Jong Shin, Chun, Jang-Soo, Yoo, Yung Joon
• Format: Article
• Language: English
• Subjects: AKR, aldo-keto reductase; Animals; CA-II, carbonic anhydrase-II; Carbazoles - pharmacology; Cell Differentiation; Cells, Cultured; Chick Embryo; Chondrocyte; Chondrocytes - cytology; Chondrocytes - drug effects; Chondrocytes - metabolism; Chondrogenesis; Chondrogenesis - drug effects; Chondrogenesis - physiology; CRABP, cellular retinoic acid binding protein; ECM, extracellular matrix; Electrophoresis, Gel, Two-Dimensional; Enzyme Inhibitors - pharmacology; Extracellular matrix; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; FABP, fatty acid binding protein; Flavonoids - pharmacology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Imidazoles - pharmacology; Indoles - pharmacology; Mesenchymal cell; Mesoderm - cytology; Mesoderm - drug effects; Mesoderm - metabolism; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases - metabolism; p38 Mitogen-Activated Protein Kinases; PAPS, 3′-phosphoadenosine 5′-phosphosulfate; PMF, peptide mass fingerprinting; Protein Kinases - metabolism; Proteins - metabolism; Proteome - analysis; Pyridines - pharmacology; Signal Transduction - drug effects; Signaling pathway; Time Factors
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(04)00243-1

We performed comparative proteome analysis of mesenchymal cells and chondrocytes to identify proteins differentially expressed during chondrogenesis. Nine such proteins were identified. Type II collagen, matrilin-1, carbonic anhydrase-II (CA-II), 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase-2, and aldo-keto reductase were increased during chondrogenesis, whereas cellular retinoic acid binding protein-I (CRABP-I), CRABP-II, cytoplasmic type 5 actin, and fatty acid binding protein were decreased or almost disappeared. Expression of type II collagen, matrilin-1, PAPS synthetase-2, and CA-II was regulated by extracellular signal-regulated protein kinase, protein kinase C, and p38 kinase, signaling molecules known to regulate chondrogenesis.