Liquid chromatographic determination of amodiaquine in human plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-04, Vol.803 (2), p.371-374
• Main Authors: Dua, Virendra K, Gupta, N.C, Sharma, V.P, Subbarao, S.K
• Format: Article
• Language: English
• Subjects: Amodiaquine; Amodiaquine - blood; Analysis; Analytical, structural and metabolic biochemistry; Antimalarials - blood; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Medical sciences; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.01.011

A normal-phase high-performance liquid chromatographic method using dichloromethane– methanol–1 M perchloric acid (100:10:0.9, v/v/v) at a flow rate of 1.0 ml min −1 on a LiChrospher Si column with UV (254 nm) detection has been developed for the determination of amodiaquine and its metabolites desethyl amodiaquine and bisdesethyl amodiaquine in plasma. The limit of quantification was 5 ng ml −1. Mean within-day and day-to-day coefficients of variation (CV) were 4.10 and 6.27% for amodiaquine, 3.43 and 4.80% for desethyl amodiaquine and 3.53 and 5.23% for bisdesethyl amodiaquine, respectively. Mean extraction recovery of amodiaquine, desethyl amodiaquine and bisdesethyl amodiaquine from plasma were 82.48, 74.50 and 69.65%, respectively. Chloroquine and its metabolite desethyl chloroquine, quinine, sulfadoxine and primaquine do not interfere in the detection of amodiaquine, desethyl amodiaquine and bisdesethyl amodiaquine in plasma.