Concanavalin A chromatography coupled to two-dimensional gel electrophoresis improves protein expression studies of the serum proteome

In the present study, we show a simple method to analyse human serum proteins using Concanavalin A (Con A) chromatography coupled to two-dimensional gel electrophoresis. Serum samples were separated into two fractions, one mainly containing non-glycosylated and O-glycosylated proteins and the other...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-04, Vol.803 (2), p.337-343
Main Authors: Rodrı́guez-Piñeiro, Ana Marı́a, Ayude, Daniel, Rodrı́guez-Berrocal, Francisco Javier, Páez de la Cadena, Marı́a
Format: Article
Language:English
Subjects:
Albumin
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, Liquid - methods
Concanavalin A
Concanavalin A - chemistry
Electrophoresis, Gel, Two-Dimensional - methods
Female
Fundamental and applied biological sciences. Psychology
General pharmacology
Glycosylation
Humans
Male
Medical sciences
Pharmacology. Drug treatments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, we show a simple method to analyse human serum proteins using Concanavalin A (Con A) chromatography coupled to two-dimensional gel electrophoresis. Serum samples were separated into two fractions, one mainly containing non-glycosylated and O-glycosylated proteins and the other enriched in N-glycosylated proteins. Both fractions were subjected to two-dimensional gel electrophoresis, and the obtained maps were analysed. The method presented here improves the resolution of the serum proteome, increasing the number of visualized spots over two times and allowing the detection of proteins with lower abundance in serum. We have proved the feasibility of the method comparing the N-glycoprotein fraction of serum from donors and colorectal cancer (CRC) patients.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2004.01.019