Marek's Disease in Turkeys. II. Characterization of the Viral Glycoprotein B Gene and Antigen of a Turkey Strain of Marek's Disease Virus

Marek's disease virus (MDV) causes immunosuppression and tumors in chickens. As sporadic cases of Marek's disease (MD) were recorded in turkeys, the antigenic and genomic characteristics of the MDV glycoprotein B (gB) gene and antigen of turkeys were compared to the chicken MDV gB. The who...

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Bibliographic Details
Published in:Avian diseases 2002-04, Vol.46 (2), p.322-333
Main Authors: Davidson, Irit, Malkinson, M, Weisman, Y
Format: Article
Language:English
Subjects:
amino acid sequences
Animals
Antibodies, Monoclonal
Antibodies, Viral - immunology
antigen
Antigens
Antigens, Viral - genetics
Antigens, Viral - immunology
Base Sequence
Central nervous system viral diseases
Chickens
DNA
DNA, Viral - chemistry
Epitopes
Epitopes - analysis
Gallid herpesvirus 2
Gene Amplification
genes
glycoproteins
Herpesvirus 2, Gallid - genetics
Herpesvirus 2, Gallid - immunology
hosts
immunoblotting
Mardivirus
Marek disease
Marek Disease - virology
Marek's disease virus
Molecular Sequence Data
monoclonal antibodies
Nervous system diseases
nucleotide sequences
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Regular s
Sequence Alignment - veterinary
tumors
Turkeys
Viral diseases
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
viral proteins
Viruses
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Summary:Marek's disease virus (MDV) causes immunosuppression and tumors in chickens. As sporadic cases of Marek's disease (MD) were recorded in turkeys, the antigenic and genomic characteristics of the MDV glycoprotein B (gB) gene and antigen of turkeys were compared to the chicken MDV gB. The whole chicken and turkey gB genes were sequenced and found identical. By immunoblotting of infected-cell culture lysates using chicken convalescent and gB monoclonal antibodies, the antigenic epitopes of the chicken and turkey viruses were found to differ. The turkey MDV had a unique epitope, compared to the chicken MDV and compared with our previous findings. While the chicken MDV had two epitope types, heat-labile but dithiothreitol (DTT)-stable and heat-stable but DTT-labile, the turkey MDV gB epitope is both heat and DTT-labile.
ISSN:0005-2086
1938-4351
DOI:10.1637/0005-2086(2002)046[0322:MSDITI]2.0.CO;2