Comparative analysis and validation of different assays for glycopeptide susceptibility among methicillin-resistant Staphylococcus aureus strains

Detection of methicillin-resistant Staphylococcus aureus (MRSA) isolates exhibiting intermediate susceptibility to glycopeptides (GISA) is challenging for clinical microbiology laboratories. We compared three different screening assays for evaluating trends in decreased glycopeptide susceptibility d...

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Bibliographic Details
Published in:Journal of microbiological methods 2004-05, Vol.57 (2), p.231-239
Main Authors: Bernard, Louis, Vaudaux, Pierre, Rohner, Peter, Huggler, Elzbieta, Armanet, Mathieu, Pittet, Didier, Lew, Daniel P., Schrenzel, Jacques
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Blood - microbiology
DNA Fingerprinting
DNA, Bacterial - analysis
DNA, Bacterial - isolation & purification
Drug Resistance, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Glycopeptide susceptibility
Humans
Methicillin Resistance
Methicillin-resistant Staphylococcus aureus strains
Microbial Sensitivity Tests - methods
Microbiology
Molecular Epidemiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymorphism, Restriction Fragment Length
Sensitivity and Specificity
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - isolation & purification
Teicoplanin - pharmacology
Vancomycin
Vancomycin - pharmacology
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Summary:Detection of methicillin-resistant Staphylococcus aureus (MRSA) isolates exhibiting intermediate susceptibility to glycopeptides (GISA) is challenging for clinical microbiology laboratories. We compared three different screening assays for evaluating trends in decreased glycopeptide susceptibility during two periods. Ninety four and ninety five consecutive MRSA blood isolates from 189 bacteremic patients collected during periods A (1989–1994) and B (1999–2001), respectively, were screened in parallel for vancomycin or teicoplanin susceptibility by glycopeptide-containing brain–heart infusion agar (BHIA) tests, Etest MICs performed at a standard (0.5 McFarland) or high (2.0 McFarland) inoculum on Mueller-Hinton (MHA) or BHIA, respectively. Any MRSA isolate yielding
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.01.012