Electrophoretically mediated microanalysis

This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-04, Vol.1032 (1), p.173-184
Main Authors: Nováková, Soňa, Van Dyck, Sigrid, Van Schepdael, Ann, Hoogmartens, Jos, Glatz, Zdeněk
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Chip technology
Chromatographic methods and physical methods associated with chromatography
Electrophoresis, Capillary - methods
Electrophoretically mediated microanalysis
Enzymes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Other chromatographic methods
Reviews
Semiconductors
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Summary:This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.
ISSN:0021-9673
DOI:10.1016/j.chroma.2003.12.025