Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool

Colorectal cancer is a common disease, and more reliable screening methods are needed for early detection. We aim to develop a non-invasive, stool-based assay that can identify colorectal cancer by detection of minichromosome maintenance protein 2 (MCM2) expression in colonocytes retrieved from the...

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Bibliographic Details
Published in:The Lancet (British edition) 2002-06, Vol.359 (9321), p.1917-1919
Main Authors: Davies, R Justin, Freeman, Alex, Morris, Lesley S, Bingham, Sheila, Dilworth, Stephen, Scott, Ian, Laskey, Ronald A, Miller, Richard, Coleman, Nicholas
Format: Article
Language:English
Subjects:
Adenocarcinoma - diagnosis
Analysis of Variance
Biological and medical sciences
Biomarkers, Tumor - analysis
Bird, John
Brown, Simon
Cancer
Case-Control Studies
Cell cycle
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - diagnosis
Data analysis
Feces - chemistry
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Hypotheses
Medical research
Medical sciences
Medical screening
Minichromosome Maintenance Complex Component 2
Nuclear Proteins - analysis
Proteins
Reproducibility of Results
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:Colorectal cancer is a common disease, and more reliable screening methods are needed for early detection. We aim to develop a non-invasive, stool-based assay that can identify colorectal cancer by detection of minichromosome maintenance protein 2 (MCM2) expression in colonocytes retrieved from the faecal surface. We devised a cell line model to investigate methods and conditions for optimum colonocyte retrieval. In our clinical evaluation study, MCM2-positive cells were retrieved from 37 of 40 patients with symptomatic colorectal cancer, but from none of 25 healthy control individuals. These results suggest that immunocytochemical analysis of retrieved colonocytes might enable accurate detection of colorectal cancer in stool.
ISSN:0140-6736
1474-547X
DOI:10.1016/S0140-6736(02)08739-1