Identification and Sequence Analysis of Six New Members of the NIMA-related Kinase Family in Chlamydomonas

The NIMA kinases are an evolutionarily conserved protein family with enigmatic roles in the regulation of mitosis. We report six new members of this family in Chlamydomonas, in addition to the previously identified NIMA-related kinase, Fa2p. ChlamydomonasNIMA-related kinases (CNKs) 1–6 were sequence...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2004-01, Vol.51 (1), p.66-72
Main Authors: BRADLEY, BRIAN A, WAGNER, JAMES J. D, QUARMBY, LYNNE M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Basal bodies
Base Sequence
Biological and medical sciences
Cell Cycle Proteins - genetics
centrioles
centrosomes
Chlamydomonas - classification
Chlamydomonas - enzymology
Chlamydomonas - genetics
cilia
CNK
Conserved Sequence
deflagellation
DNA Primers
Fa2
flagella
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic
General aspects and techniques
microtubules
Molecular Sequence Data
Nek
NIMA-Related Kinase 1
Phylogeny
Protein-Serine-Threonine Kinases - genetics
Protozoa
Protozoan Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The NIMA kinases are an evolutionarily conserved protein family with enigmatic roles in the regulation of mitosis. We report six new members of this family in Chlamydomonas, in addition to the previously identified NIMA-related kinase, Fa2p. ChlamydomonasNIMA-related kinases (CNKs) 1–6 were sequenced from subclones generated by RT-PCR using information from EST libraries and the recently sequenced Chlamydomonas genome. Phylogenetic and bioinformatic approaches were used to determine the relationships of the six new members with known members of the NIMA-related kinase family. Although humans express at least eleven NIMA-related kinases, the eukaryotic microbes that have been studied to date express only one or two members of the family. Thus, the discovery that Chlamydomonas expresses a total of at least seven NIMA-related kinases is intriguing. Our analyses suggest that members of this family may play roles in the assembly and function of cilia.
ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.2004.tb00164.x