Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-1 (E-NPP1/PC-1) and -3 (E-NPP3/CD203c/PD-Iβ/B10/gp130 RB13-6) in inflammatory and neoplastic bile duct diseases

• Published in: Cancer letters 2004-04, Vol.207 (2), p.139-147
• Main Authors: Yano, Yoshihiko, Hayashi, Yoshitake, Sano, Kimihiko, Nagano, Hidenobu, Nakaji, Miyuki, Seo, Yasushi, Ninomiya, Toshiaki, Yoon, Seitetsu, Yokozaki, Hiroshi, Kasuga, Masato
• Format: Article
• Language: English
• Subjects: Animals; Autoimmune diseases; Bile; Bile duct carcinoma; Bile Duct Neoplasms - enzymology; Bile Duct Neoplasms - genetics; Bile Duct Neoplasms - pathology; Blotting, Western; Carcinogenesis; Case-Control Studies; Cell Membrane - enzymology; Cell Movement; Cytoplasm - metabolism; DNA, Complementary - genetics; DNA, Complementary - metabolism; E-NPP1; E-NPP3; Humans; Immunoenzyme Techniques; In Situ Hybridization; Liver Cirrhosis, Biliary - enzymology; Liver Cirrhosis, Biliary - genetics; Liver Cirrhosis, Biliary - pathology; Mice; Motility; NIH 3T3 Cells; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - metabolism; Primary biliary cirrhosis; Proteins; Pyrophosphatases - genetics; Pyrophosphatases - metabolism; RNA Probes; Rodents; Transfection
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2003.11.002

Ecto-nucleotide pyrophosphatase/phosphodiesterase-I enzyme (E-NPP) consists of three closely related molecules: E-NPP1, E-NPP2 and E-NPP3. We investigated the expression and localization of E-NPP1 and -3 in human inflammatory and neoplastic bile duct diseases. Immunohistochemically E-NPP1 was located on the apical cytoplasmic side of cancer cells, whereas E-NPP3 was located in the apical plasma membrane. Western blot analysis revealed that the expression of E-NPP3, but not E-NPP1, was higher in tumor tissues than in surrounding tissues and the specific form of the E-NPP3 protein was readily detected in the sera of bile duct carcinoma (BDC) patients. Furthermore, it was confirmed that E-NPP3 was associated with migration ability by using of NIH3T3 cells that stably transfected with E-NPP3 cDNA. These results suggest that E-NPP3 is involved in the infiltration of neoplastic BDC and is possible to be a tumor marker.