Structure of the phenol-soluble polysaccharide from Shewanella putrefaciens strain A6

• Published in: Carbohydrate research 2002-06, Vol.337 (12), p.1119-1127
• Main Authors: Shashkov, Alexander S, Torgov, Vladimir I, Nazarenko, Evgeny L, Zubkov, Vladimir A, Gorshkova, Natalya M, Gorshkova, Raisa P, Widmalm, Göran
• Format: Article
• Language: English
• Subjects: Biosynthesis; Magnetic Resonance Spectroscopy; Molecular Structure; NMR spectroscopy; Nonulosonic acid; Phenol - chemistry; Polysaccharide; Polysaccharides - chemistry; Shewanella putrefaciens; Shewanella putrefaciens - chemistry; Shewanellose; Solubility
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/S0008-6215(02)00101-5

The structure of the phenol-soluble polysaccharide from Shewanella putrefaciens strain A6 has been elucidated. Chemical modifications of the polymer in conjunction with 1H and 13C NMR spectroscopy, including 2D techniques, were employed in the analysis. It is concluded that the repeating unit is composed of two nine-carbon sugars as follows: →4)-α-Non pA-(2→3)-β-Sug p-(1→ where α-Non pA is 5-acetamido-7-acetamidino-8- O-acetyl-3,5,7,9-tetradeoxy- l- glycero-α- d- galacto-non-2-ulosonic acid (8eLeg) and β-Sug p is 2-acetamido-2,6-dideoxy-4- C-(3′-carboxamide-2′,2′-dihydroxypropyl)-β- d-galactopyranose, with the proposed name Shewanellose (She). The repeating unit is composed of two nine-carbon sugars: →4)-α-Non pA-(2→3)-β-Sug p-(1→ where α-Non pA is 5-acetamido-7-acetamidino-8- O-acetyl-3,5,7,9-tetradeoxy- l- glycero-α- d- galacto-non-2-ulosonic acid (8eLeg) and β-Sug p is 2-acetamido-2,6-dideoxy-4- C-(3′-carboxamide-2′,2′-dihydroxypropyl)-β- d-galactopyranose.