Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution

• Published in: FEBS letters 2002-04, Vol.517 (1), p.19-23
• Main Authors: Cumino, Andrea, Curatti, Leonardo, Giarrocco, Laura, Salerno, Graciela L.
• Format: Article
• Language: English
• Subjects: An7119-, Anabaena sp. PCC7119; Anabaena; Anabaena - enzymology; At-, Arabidopsis thaliana; Cyanobacteria - enzymology; Cyanobacterium; Evolution, Molecular; Glucosyltransferases - chemistry; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; GTD, glucosyltransferase domain; Modular arrangement; Molecular Sequence Data; Mt-, Medicago truncatula; Np-, Nostoc punctiforme; Os-, Oryza sativa; PHD, phosphohydrolase domain; phosphatase; phosphohydrolase; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; Phosphoric Monoester Hydrolases - chemistry; Phylogenetic analysis; Phylogeny; Plants - enzymology; PmMED-, Prochlorococcus marinus MED4; PmMIT-, P. marinus MIT9313; SBP, sucrose biosynthesis-related proteins; Sequence Alignment; Sequence Analysis, Protein; Sm-, Synechococcus marinus WH8102; SPP, sucrose-phosphate phosphatase; SPS, sucrose-phosphate synthase; SPS-like gene; St-, Solanum tuberosum; Sucrose - metabolism; Sucrose synthase; Sucrose-phosphate phosphatase; Sucrose-phosphate synthase; SuS, sucrose synthase; Sy-, Synechocystis sp. PCC6803; The prefixes indicate: An-, Anabaena sp. PCC7120; Zm-, Zea mays
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(02)02516-4

Based on the functional characterization of sucrose biosynthesis related proteins [SBP: sucrose-phosphate synthase (SPS), sucrose-phosphate phosphatase (SPP), and sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture. Anabaena SPS, a minimal catalytic SPS unit, defines a glucosyltransferase domain present in all SPSs and SuSs. Similarly, Anabaena SPP defines a phosphohydrolase domain characteristic of all SPPs and some SPSs. Phylogenetic analysis points towards the evolution of modern cyanobacterial and plant SBP from a bidomainal common ancestral SPS-like gene.