Detection of the initial infective stages of the protozoan parasite Marteilia sydneyi in Saccostrea glomerata and their development through to sporogenesis

DNA probes were used in in situ hybridisation on histological sections of oysters exposed for defined intervals to Marteilia sydneyi infection to reveal the early development of the parasite in the oyster host, Saccostrea glomerata. The initial infective stages enter through the palps and gills wher...

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Bibliographic Details
Published in:International journal for parasitology 2002-06, Vol.32 (6), p.767-784
Main Authors: Kleeman, S.N, Adlard, R.D, Lester, R.J.G
Format: Article
Language:English
Subjects:
Animals
Australia
Biological and medical sciences
Disease development
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
Eukaryota - genetics
Eukaryota - growth & development
Eukaryota - ultrastructure
Fundamental and applied biological sciences. Psychology
In situ hybridisation
In Situ Hybridization - veterinary
Life cycle
Life cycle. Host-agent relationship. Pathogenesis
Marine
Marteilia sydneyi
Microscopy, Electron - veterinary
Ostreidae - parasitology
Oyster
Paramyxea
Protozoa
Protozoan Infections, Animal - parasitology
Saccostrea glomerata
Statistics, Nonparametric
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Summary:DNA probes were used in in situ hybridisation on histological sections of oysters exposed for defined intervals to Marteilia sydneyi infection to reveal the early development of the parasite in the oyster host, Saccostrea glomerata. The initial infective stages enter through the palps and gills whereupon extrasporogonic proliferation results in the liberation of cells into surrounding connective tissue and haemolymph spaces. Following systemic dissemination, the parasite infiltrates the digestive gland and becomes established as a nurse cell beneath the epithelial cells in a digestive tubule. Here, cell-within-cell proliferation results in the eventual liberation of daughter cells from the nurse cell into spaces between adjacent epithelial cells. None of these stages had previously been described. Proliferation is associated with host responses, including haemocytic infiltration of the connective tissue and diapedesis across tubule epithelia. The responses cease as sporogenesis begins.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(02)00025-5