Diurnal rhythm of H+-peptide cotransporter in rat small intestine

In mammals, most physiological, biochemical, and behavioral processes show a circadian rhythm. In the present study, we examined the diurnal rhythm of the H+-peptide cotransporter (PEPT1), which transports small peptides and peptide-like drugs in the small intestine and kidney, using rats maintained...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 2002-07, Vol.283 (1), p.G57-G64
Main Authors: Pan, Xiaoyue, Terada, Tomohiro, Irie, Megumi, Saito, Hideyuki, Inui, Ken-Ichi
Format: Article
Language:English
Subjects:
Absorption
Animals
Carrier Proteins - genetics
Carrier Proteins - metabolism
Circadian Rhythm
Dipeptides - pharmacokinetics
Duodenum - metabolism
In Vitro Techniques
Kidney - metabolism
Male
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
Peptide Transporter 1
Photoperiod
Rats
Rats, Wistar
RNA, Messenger - metabolism
Sodium-Glucose Transporter 1
Symporters - genetics
Symporters - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In mammals, most physiological, biochemical, and behavioral processes show a circadian rhythm. In the present study, we examined the diurnal rhythm of the H+-peptide cotransporter (PEPT1), which transports small peptides and peptide-like drugs in the small intestine and kidney, using rats maintained in a 12-h photoperiod with free access to chow. The transport of [14C]glycylsarcosine (Gly-Sar), a typical substrate for PEPT1 by in situ intestinal loop and everted intestine, was greater in the dark phase than the light phase. PEPT1 protein and mRNA levels varied significantly, with a maximum at 2000 and minimum at 800. Similar functional and expressional diurnal variations were observed in the intestinal Na+-glucose cotransporter (SGLT1). In contrast, renal PEPT1 and SGLT1 showed little diurnal rhythmicity in protein and mRNA expression. These findings indicate that the intestinal PEPT1 undergoes diurnal regulation in its activity and expression, and this could affect the intestinal absorption of dietary protein.
ISSN:0193-1857
1522-1547
DOI:10.1152/ajpgi.00545.2001