Metabolic control analysis of anaerobic glycolysis in human hibernating myocardium replaces traditional concepts of flux control

• Published in: FEBS letters 2002-04, Vol.517 (1), p.245-250
• Main Authors: Vogt, Achim M., Nef, Holger, Schaper, Jutta, Poolman, Mark, Fell, David A., Kübler, Wolfgang, Elsässer, Albrecht
• Format: Article
• Language: English
• Subjects: 2PG, 2-phosphoglyceric acid; Anaerobiosis - physiology; Biopsy; DHAP, dihydroxyacetone phosphate; Energy metabolism; Eno, enolase; F1,6P2, fructose 1,6-bisphosphate; F6P, fructose 6-phosphate; G6P, glucose 6-phosphate; GA3P, glyceraldehyde 3-phosphate; GAPDH, glyceraldehyde dehydrogenase; Glucose - metabolism; Glycogen - metabolism; Glycolysis; Glycolysis - physiology; GP-iso, glucosephosphate isomerase; Hibernating myocardium; Humans; Ischemia; Lac, lactate; LDH, lactate dehydrogenase; Metabolic regulation; Myocardial Stunning - blood; Myocardial Stunning - metabolism; PEP, phosphoenolpyruvate; Perfusion; PFK-1, phosphofructokinase 1; PGK, phosphoglycerate kinase; PGM, phosphoglycerate mutase; PK, pyruvate kinase; Pyr, pyruvate
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(02)02632-7

Myocardial hibernation represents an adaptation to sustained ischemia to maintain tissue vitality during severe supply–demand imbalance which is characterized by an increased glucose uptake. To elucidate this adaptive protective mechanism, the regulation of anaerobic glycolysis was investigated using human biopsies. In hibernating myocardium showing an increase in anaerobic glycolytic flux metabolizing exogenous glucose, the adjustment of flux through this pathway was analyzed by flux:metabolite co-responses. By this means, a previously unknown pattern of regulation using multisite modulation was found which largely differs from traditional concepts of metabolic control of the Embden–Meyerhof pathway in normal and diseased myocardium.