OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis

OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly i...

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Bibliographic Details
Published in:Journal of neuroimmunology 2004-05, Vol.150 (1), p.186-198
Main Authors: Carlson, Kimberly A., Limoges, Jenae, Pohlman, Garrett D., Poluektova, Larisa Y., Langford, Dianne, Masliah, Eliezer, Ikezu, Tsuneya, Gendelman, Howard E.
Format: Article
Language:English
Subjects:
AIDS Dementia Complex - immunology
AIDS Dementia Complex - metabolism
AIDS Dementia Complex - pathology
Brain - cytology
Brain - immunology
Brain - metabolism
Cells, Cultured
Cytomegalovirus
Disease markers
Disease Progression
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Encephalitis, Viral - immunology
Encephalitis, Viral - metabolism
Encephalitis, Viral - pathology
Gene Expression Regulation - immunology
HIV-1 - immunology
HIV-1 encephalitis
Human immunodeficiency virus 1
Humans
Macrophages
Macrophages - immunology
Macrophages - metabolism
Macrophages - virology
Microglia
Organ Specificity - genetics
Organ Specificity - immunology
Severity of Illness Index
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcriptional factor
Virus Replication - genetics
Virus Replication - immunology
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Description
Summary:OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2004.01.021