Clustering of a lipid-raft associated pool of ERM proteins at the immunological synapse upon T cell receptor or CD28 ligation

Although ezrin is tyrosine phosphorylated following TCR ligation, its biological role in T cell activation is not known. Here, we shhow that ezrin clusters at the immunological synapse upon T cell stimulation. Clustering of ezrin can be triggered by TCR ligation, or, more efficiently, by CD28 ligati...

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Bibliographic Details
Published in:Immunology letters 2002-09, Vol.83 (2), p.143-147
Main Authors: Tomas, Elizabeth M., Chau, Thu A., Madrenas, Joaquı́n
Format: Article
Language:English
Subjects:
CD28 Antigens - immunology
CD28 Antigens - metabolism
Cytoskeletal Proteins
DNA-Binding Proteins - immunology
DNA-Binding Proteins - metabolism
Ezrin
Humans
Immunological synapse
Jurkat Cells
Lipid rafts
Membrane Microdomains - metabolism
Microscopy, Confocal
Phosphoproteins - metabolism
Phosphorylation
Receptors, Antigen, T-Cell - immunology
Receptors, Antigen, T-Cell - metabolism
Transcription Factors - immunology
Transcription Factors - metabolism
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Summary:Although ezrin is tyrosine phosphorylated following TCR ligation, its biological role in T cell activation is not known. Here, we shhow that ezrin clusters at the immunological synapse upon T cell stimulation. Clustering of ezrin can be triggered by TCR ligation, or, more efficiently, by CD28 ligation. The clusters of ezrin at the immunological synapse include serine/threonine phosphorylated ezrin predominantly located within cell membrane lipid rafts. Based on these data, we propose that ezrin may play a role in the formation/stabilization of lipid raft signalosomes at the immunological synapse and therefore contribute to sustain TCR-dependent signalling.
ISSN:0165-2478
1879-0542
DOI:10.1016/S0165-2478(02)00075-5