Cyanidin and Malvidin from Oryza sativa cv. Heugjinjubyeo mediate cytotoxicity against human monocytic leukemia cells by arrest of G(2)/M phase and induction of apoptosis

Oryza sativa cv. Heugjinjubyeo (Gramineae), anthocyanin-pigmented rice, having dark purple grains, is known broadly as enriched rice with an improved taste. Two bioactive compounds were isolated from the 0.5% HCl-ethyl alcohol soluble fraction of the aleurone layer of O. sativa cv. Heugjinjubyeo thr...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2004-04, Vol.52 (8), p.2213-2217
Main Authors: Hyun, Jin Won, Chung, Ha Sook
Format: Article
Language:English
Subjects:
Anthocyanins - isolation & purification
Anthocyanins - pharmacology
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Cell Cycle - drug effects
G2 Phase - drug effects
Humans
Mitosis - drug effects
Oryza - chemistry
Tumor Cells, Cultured
U937 Cells
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Summary:Oryza sativa cv. Heugjinjubyeo (Gramineae), anthocyanin-pigmented rice, having dark purple grains, is known broadly as enriched rice with an improved taste. Two bioactive compounds were isolated from the 0.5% HCl-ethyl alcohol soluble fraction of the aleurone layer of O. sativa cv. Heugjinjubyeo through an activity-monitored fractionation and isolation method. From spectral analysis, the cytotoxic components were the anthocyanidins cyanidin (1) and malvidin (2) The 50% growth inhibitory concentrations (IC(50)) of cyanidin and malvidin on U937, human monocytic leukemia cells, were 60 and 40 microg/mL, respectively. These compounds showed cytotoxicity through the arrest of the G(2)/M phase of cell cycle and induction of apoptosis.
ISSN:0021-8561
DOI:10.1021/jf030370h