Pharmacology of skin aging. Stimulation of glycosaminoglycan biosynthesis by L-fucose and fucose-rich polysaccharides, effect of in vitro aging of fibroblasts

The effect of L-fucose and fucose-rich polysaccharides (FROP-s [Biomed. Pharmacother., 2003; 57: 187–94]) was investigated, using human skin fibroblast cultures at several passages. The cells were incubated with radioactive glucosamine for 24 h, followed by the determination of individual glycosamin...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2004-04, Vol.58 (3), p.202-204
Main Authors: Isnard, N., Fodil-Bourahla, I., Robert, A.M., Robert, L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cells, Cultured
Dermatan sulfate
Fibroblasts - drug effects
Fibroblasts - metabolism
Fucose - analogs & derivatives
Fucose - pharmacology
Fucose-rich polysaccharide
Glycosaminoglycan
Glycosaminoglycans - biosynthesis
Heparan sulfate
Humans
Hyaluronan
Keratan sulfate
L-Fucose
Matrix biosynthesis
Medical sciences
Pharmacology. Drug treatments
Polysaccharides - pharmacology
Skin Aging - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of L-fucose and fucose-rich polysaccharides (FROP-s [Biomed. Pharmacother., 2003; 57: 187–94]) was investigated, using human skin fibroblast cultures at several passages. The cells were incubated with radioactive glucosamine for 24 h, followed by the determination of individual glycosaminoglycans (GAG-s) by selective hydrolysis using specific enzymes. The effect of L-fucose and of FROP-3 [Biomed. Pharmacother., in 2003; 57: 187–94], both at 1 and 10 μg/ml, added to the culture medium, was investigated. L-Fucose stimulated the incorporation of the tracer in heparan sulfates by fibroblasts at the ninth passage by 20%. FROP-3 stimulated incorporation in keratan sulfates by 45% by fibroblasts of the fifth passage. This effect was identical at both concentrations tested. For fibroblasts at the ninth passage FROP-3 stimulated incorporation in dermatan sulfate. This effect was dose dependent of the order of +67% at 1 μg/ml and +128% at 10 μg/ml. Incorporation in hyaluronan was also stimulated by about +27%. These stimulations of GAG-biosynthesis might play a role in the increase of total skin thickness of hairless rats treated with L-fucose, as well as in several other favorable results recorded for FROP-3 such as the increased hydration (resistance to pressure) and elasticity of human skin (Robert C, Robert AM, Robert L).
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2003.07.002