Introduction of Functional Groups onto Polypropylene and Polyethylene Surfaces for Immobilization of Enzymes

Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37°C. When untreated...

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Bibliographic Details
Published in:Analytical biochemistry 2002-07, Vol.306 (1), p.74-78
Main Authors: Naqvi, Azmi, Nahar, Pradip, Gandhi, R.P.
Format: Article
Language:English
Subjects:
1-fluoro-2-nitro-4-azidobenzene
covalent immobilization
Enzyme Stability
Enzymes, Immobilized - metabolism
glucose oxidase
Glucose Oxidase - metabolism
horseradish peroxidase
Horseradish Peroxidase - metabolism
photoactivation
polyethylene
Polyethylenes - chemistry
polypropylene
Polypropylenes - chemistry
Protein Binding
Surface Properties
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Summary:Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37°C. When untreated surfaces are used, insignificant immobilization of the enzymes is observed.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2002.5675