Period2::Luciferase Real-Time Reporting of Circadian Dynamics Reveals Persistent Circadian Oscillations in Mouse Peripheral Tissues

Mammalian circadian rhythms are regulated by the suprachiasmatic nucleus (SCN), and current dogma holds that the SCN is required for the expression of circadian rhythms in peripheral tissues. Using a PERIOD2::LUCIFERASE fusion protein as a real-time reporter of circadian dynamics in mice, we report...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2004-04, Vol.101 (15), p.5339-5346
Main Authors: Yoo, Seung-Hee, Yamazaki, Shin, Lowrey, Phillip L., Shimomura, Kazuhiro, Ko, Caroline H., Buhr, Ethan D., Siepka, Sandra M., Hong, Hee-Kyung, Oh, Won Jun, Yoo, Ook Joon, Menaker, Michael, Takahashi, Joseph S.
Format: Article
Language:English
Subjects:
Alleles
Animals
Biological Sciences
Bioluminescence
Circadian rhythm
Circadian Rhythm - genetics
Circadian Rhythm - physiology
Cornea
Cornea - physiology
Culture Techniques
Female
Kidney - physiology
Kidneys
Lesions
Liver
Liver - physiology
Luciferases - genetics
Luciferases - metabolism
Luminescent Measurements
Lung - physiology
Lungs
Male
Mammals
Mice
Mice, Inbred C57BL
Mice, Transgenic
Motor Activity - physiology
Neurology
Nuclear Proteins - genetics
Nuclear Proteins - physiology
Oscillators
Phenotype
Pituitary Gland - physiology
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - physiology
Suprachiasmatic Nucleus - physiology
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Summary:Mammalian circadian rhythms are regulated by the suprachiasmatic nucleus (SCN), and current dogma holds that the SCN is required for the expression of circadian rhythms in peripheral tissues. Using a PERIOD2::LUCIFERASE fusion protein as a real-time reporter of circadian dynamics in mice, we report that, contrary to previous work, peripheral tissues are capable of self-sustained circadian oscillations for > 20 cycles in isolation. In addition, peripheral organs expressed tissue-specific differences in circadian period and phase. Surprisingly, lesions of the SCN in mPer2Luciferase knockin mice did not abolish circadian rhythms in peripheral tissues, but instead caused phase desynchrony among the tissues of individual animals and from animal to animal. These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0308709101