Use of Chimeric Fluorescent Proteins and Fluorescence Resonance Energy Transfer to Monitor Cellular Responses

ABSTRACT—In recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize indivi...

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Bibliographic Details
Published in:Circulation research 2004-04, Vol.94 (7), p.866-873
Main Authors: Zaccolo, Manuela, O’Rourke, Brian
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - analysis
Bacterial Proteins - chemistry
Biological and medical sciences
Fluorescence Resonance Energy Transfer - methods
Fluorescent Dyes - analysis
Forecasting
Fundamental and applied biological sciences. Psychology
Green Fluorescent Proteins
Humans
Luminescent Proteins - analysis
Luminescent Proteins - chemistry
Luminescent Proteins - genetics
Microscopy, Fluorescence
Models, Cardiovascular
Myocytes, Cardiac - metabolism
Myocytes, Cardiac - ultrastructure
Protein Conformation
Protein Interaction Mapping
Recombinant Fusion Proteins - analysis
Signal Transduction
Vertebrates: cardiovascular system
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Summary:ABSTRACT—In recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize individual molecular events as they happen in intact, live cells, providing important and original insights for our understanding of biologically relevant problems. This review discusses some essential methodological aspects concerning the generation and use of fluorescence resonance energy transfer–based biosensors and presents selected examples of specific applications that highlight the power of this technology.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.0000123825.83803.CD