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Amino acids control ammonia pulses in yeast colonies
Individual yeast colonies produce pulses of volatile ammonia separated by phases of medium acidification. Colonies of Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the S. cerevisiae amino acid sensor SPS comple...
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| Published in: | Biochemical and biophysical research communications 2002-06, Vol.294 (5), p.962-967 |
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| Main Authors: | , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c392t-69e15309e77da901662452bef2269a4c9b348ba0d642f7ec740762ce4c9d2b8c3 |
| container_end_page | 967 |
| container_issue | 5 |
| container_start_page | 962 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 294 |
| creator | Zikánová, Blanka Kuthan, Martin Řičicová, Markéta Forstová, Jitka Palková, Zdena |
| description | Individual yeast colonies produce pulses of volatile ammonia separated by phases of medium acidification. Colonies of
Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the
S. cerevisiae amino acid sensor SPS completely abolish the colony ammonia pulses. In contrast, the ammonia pulse production is independent of external concentrations of ammonium and of its uptake by the ammonium permeases Mep1p, Mep2p, and Mep3p. It is concluded that in
S. cerevisiae colonies, the extracellular amino acids, but not the extracellular ammonium, serve as a source for volatile ammonia production. These phenomena are not restricted to
S. cerevisiae, since we observe that extracellular levels of 8 out of the 20 tested amino acids are necessary for ammonia pulses produced by
Candida mogii colonies. |
| doi_str_mv | 10.1016/S0006-291X(02)00589-2 |
| format | article |
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Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the
S. cerevisiae amino acid sensor SPS completely abolish the colony ammonia pulses. In contrast, the ammonia pulse production is independent of external concentrations of ammonium and of its uptake by the ammonium permeases Mep1p, Mep2p, and Mep3p. It is concluded that in
S. cerevisiae colonies, the extracellular amino acids, but not the extracellular ammonium, serve as a source for volatile ammonia production. These phenomena are not restricted to
S. cerevisiae, since we observe that extracellular levels of 8 out of the 20 tested amino acids are necessary for ammonia pulses produced by
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Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the
S. cerevisiae amino acid sensor SPS completely abolish the colony ammonia pulses. In contrast, the ammonia pulse production is independent of external concentrations of ammonium and of its uptake by the ammonium permeases Mep1p, Mep2p, and Mep3p. It is concluded that in
S. cerevisiae colonies, the extracellular amino acids, but not the extracellular ammonium, serve as a source for volatile ammonia production. These phenomena are not restricted to
S. cerevisiae, since we observe that extracellular levels of 8 out of the 20 tested amino acids are necessary for ammonia pulses produced by
Candida mogii colonies.</description><subject>Amino acid permeases</subject><subject>Amino Acid Transport Systems - genetics</subject><subject>Amino Acid Transport Systems - physiology</subject><subject>Amino Acids - pharmacology</subject><subject>Ammonia - metabolism</subject><subject>Ammonia signalling</subject><subject>Ammonium permeases</subject><subject>Biological Transport</subject><subject>Candida - cytology</subject><subject>Candida - growth & development</subject><subject>Candida - metabolism</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - physiology</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - physiology</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>SPS sensor</subject><subject>Yeast colonies</subject><subject>Yeasts - growth & development</subject><subject>Yeasts - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKxDAUhoMoOo4-gtKV6KJ6kqZJs5Jh8AYDLlRwF9L0FCJtMzatMG9v5oIuZ3Ug_3cu-Qi5oHBLgYq7NwAQKVP08xrYDUBeqJQdkAkFBSmjwA_J5A85IachfAFQyoU6JieUgeS5hAnhs9Z1PjHWVSGxvht63ySmbX3nTLIcm4AhcV2yQhOGmDfxHcMZOapNjM53dUo-Hh_e58_p4vXpZT5bpDZTbEiFQppnoFDKyqh4tGA8ZyXWjAlluFVlxovSQCU4qyVayUEKZjEmFSsLm03J1XbusvffI4ZBty5YbBrToR-DlrTIVPz6XpAWnEngLIL5FrS9D6HHWi9715p-pSnotVe98arX0jQwvfGq132XuwVj2WL137UTGYH7LYDRx4_DXgfrsLNYuR7toCvv9qz4BQtEhj4</recordid><startdate>20020628</startdate><enddate>20020628</enddate><creator>Zikánová, Blanka</creator><creator>Kuthan, Martin</creator><creator>Řičicová, Markéta</creator><creator>Forstová, Jitka</creator><creator>Palková, Zdena</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20020628</creationdate><title>Amino acids control ammonia pulses in yeast colonies</title><author>Zikánová, Blanka ; 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Colonies of
Saccharomyces cerevisiae mutant defective in the general amino acid permease, Gap1p, exhibit decreased ammonia production. Mutations in the
S. cerevisiae amino acid sensor SPS completely abolish the colony ammonia pulses. In contrast, the ammonia pulse production is independent of external concentrations of ammonium and of its uptake by the ammonium permeases Mep1p, Mep2p, and Mep3p. It is concluded that in
S. cerevisiae colonies, the extracellular amino acids, but not the extracellular ammonium, serve as a source for volatile ammonia production. These phenomena are not restricted to
S. cerevisiae, since we observe that extracellular levels of 8 out of the 20 tested amino acids are necessary for ammonia pulses produced by
Candida mogii colonies.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12074570</pmid><doi>10.1016/S0006-291X(02)00589-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2002-06, Vol.294 (5), p.962-967 |
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| language | eng |
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| source | Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list) |
| subjects | Amino acid permeases Amino Acid Transport Systems - genetics Amino Acid Transport Systems - physiology Amino Acids - pharmacology Ammonia - metabolism Ammonia signalling Ammonium permeases Biological Transport Candida - cytology Candida - growth & development Candida - metabolism Carrier Proteins - genetics Carrier Proteins - physiology Intracellular Signaling Peptides and Proteins Membrane Proteins - genetics Membrane Proteins - physiology Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins SPS sensor Yeast colonies Yeasts - growth & development Yeasts - metabolism |
| title | Amino acids control ammonia pulses in yeast colonies |
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