Biotransformation of halophenols using crude cell extracts of Pseudomonas putida F6

Crude cell extracts of Pseudomonas putida F6 transformed 4-substituted fluoro-, chloro-, bromo- and iodo-phenol without the exogenous addition of cofactors. The rate of substrate consumption decreased with increasing substituent size (F>Cl>Br>I). Biotransformations resulted in greater than...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2004-05, Vol.64 (4), p.486-492
Main Authors: Brooks, S. J, Doyle, E. M, Hewage, C, Malthouse, J. P. G, Duetz, W, O’ Connor, K. E
Format: Article
Language:English
Subjects:
ascorbic acid
Bacteria
Biological and medical sciences
Biotechnology
Biotransformation
catechol
catechols
Catechols - analysis
Chlorophenols - metabolism
Coenzymes
Fundamental and applied biological sciences. Psychology
Iodobenzenes - metabolism
Microbiology
phenol
Phenols
Phenols - metabolism
Pseudomonas putida
Pseudomonas putida - growth & development
Pseudomonas putida - metabolism
Substrate Specificity
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Summary:Crude cell extracts of Pseudomonas putida F6 transformed 4-substituted fluoro-, chloro-, bromo- and iodo-phenol without the exogenous addition of cofactors. The rate of substrate consumption decreased with increasing substituent size (F>Cl>Br>I). Biotransformations resulted in greater than 95% utilisation of the halogenated substrate. Product accumulation was observed in incubations with 4-chloro, 4-bromo- and 4-iodo-phenol. These products were identified as the corresponding 4-substituted catechols. Transformation of 4-fluorophenol did not result in the accumulation of the corresponding catechol; however, manipulation of the reaction conditions by incorporation of ascorbic acid culminated in the formation of 4-fluorocatechol. Cell extracts of P. putida F6 also showed activity towards a 3-substituted phenol, namely 3-fluorophenol, resulting in the formation of a single product, 4-fluorocatechol.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-003-1488-z