The Short‐Term Effects of Treatment of Chronic Periodontitis on Circulating Levels of Endotoxin, C‐Reactive Protein, Tumor Necrosis Factor‐α, and Interleukin‐6

• Published in: Journal of periodontology (1970) 2004-03, Vol.75 (3), p.420-428
• Main Authors: Ide, Mark, Jagdev, Daljit, Coward, Paula Y., Crook, Martin, Barclay, G. Robin, Wilson, Ron F.
• Format: Article
• Language: English
• Subjects: Acute‐phase proteins; Adult; Analysis of Variance; Antibodies; C-Reactive Protein - analysis; Chromogenic Compounds; Chronic Disease; clinical trials; C‐reactive proteins; Dental Plaque Index; Dental Scaling; Dentistry; Endotoxins - blood; Female; Follow-Up Studies; Humans; Interleukin-6 - blood; interleukin‐6; Limulus Test; Lipopolysaccharides - blood; Male; Periodontal Index; Periodontitis - blood; Periodontitis - therapy; scaling; Statistics, Nonparametric; tumor necrosis factor; Tumor Necrosis Factor-alpha - analysis
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2004.75.3.420

Background: The acute‐phase response involves molecules including tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6), and C‐reactive protein (CRP). This study aimed to determine whether subgingival scaling resulted in rapid changes in plasma concentrations of these molecules. Methods: Twenty‐three non‐smoking adults with chronic periodontitis received subgingival scaling for 60 minutes. Venous blood samples were taken at 0, 15, 30, 60, and 120 minutes. TNF‐ α and IL‐6 were assayed from all samples and CRP from the baseline and final samples. Lipopolysaccharide (LPS) was assayed at 0, 15, and 30 minutes using limulus lysate assay (LAL) and EndoCAb Ig assays. Results: LPS assays were suggestive of a transient low‐grade bacteremia, but changes in LPS approaching significance (P = 0.061) were seen with LAL only. There was a significant increase in circulating TNF‐α (P = 0.0387) and IL‐6 (P