Sp1 is involved in the transcriptional activation of p16(INK4) by p21(Waf1) in HeLa cells

Both p16(INK4) and p21(Waf1) are very important negative regulators of the cell cycle. In this study we examined the effects of p21(Waf1) on the transcription of p16(INK4). We determined that p21(Waf1) can activate the transcription of p16(INK4), and that this effect is GC-box dependent. We also fou...

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Bibliographic Details
Published in:FEBS letters 2004-04, Vol.564 (1-2), p.199-204
Main Authors: Xue, Lixiang, Wu, Junfeng, Zheng, Wenjie, Wang, Peichang, Li, Jun, Zhang, Zongyu, Tong, Tanjun
Format: Article
Language:English
Subjects:
Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis
Cyclin-Dependent Kinase Inhibitor p16 - genetics
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - genetics
Cyclins - physiology
HeLa Cells
Humans
Mutation
Promoter Regions, Genetic - genetics
Sp1 Transcription Factor - genetics
Sp1 Transcription Factor - physiology
Transcriptional Activation
Transfection
Tumor Suppressor Protein p14ARF
Up-Regulation
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Summary:Both p16(INK4) and p21(Waf1) are very important negative regulators of the cell cycle. In this study we examined the effects of p21(Waf1) on the transcription of p16(INK4). We determined that p21(Waf1) can activate the transcription of p16(INK4), and that this effect is GC-box dependent. We also found that the transcription factor Sp1 plays a key role in this event. Upregulation of Sp1 contributes to the transcriptional activation and protein level of p16(INK4) mediated by p21(Waf1), and is a potential point of cooperation between the p16/pRb and p14 (ARF)/p53 tumor suppressor pathways.
ISSN:0014-5793
DOI:10.1016/S0014-5793(04)00352-7