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Sp1 is involved in the transcriptional activation of p16(INK4) by p21(Waf1) in HeLa cells

Both p16(INK4) and p21(Waf1) are very important negative regulators of the cell cycle. In this study we examined the effects of p21(Waf1) on the transcription of p16(INK4). We determined that p21(Waf1) can activate the transcription of p16(INK4), and that this effect is GC-box dependent. We also fou...

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Published in:	FEBS letters 2004-04, Vol.564 (1-2), p.199-204
Main Authors:	Xue, Lixiang, Wu, Junfeng, Zheng, Wenjie, Wang, Peichang, Li, Jun, Zhang, Zongyu, Tong, Tanjun
Format:	Article
Language:	English
Subjects:	Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis Cyclin-Dependent Kinase Inhibitor p16 - genetics Cyclin-Dependent Kinase Inhibitor p21 Cyclins - genetics Cyclins - physiology HeLa Cells Humans Mutation Promoter Regions, Genetic - genetics Sp1 Transcription Factor - genetics Sp1 Transcription Factor - physiology Transcriptional Activation Transfection Tumor Suppressor Protein p14ARF Up-Regulation
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container_title	FEBS letters
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creator	Xue, Lixiang Wu, Junfeng Zheng, Wenjie Wang, Peichang Li, Jun Zhang, Zongyu Tong, Tanjun
description	Both p16(INK4) and p21(Waf1) are very important negative regulators of the cell cycle. In this study we examined the effects of p21(Waf1) on the transcription of p16(INK4). We determined that p21(Waf1) can activate the transcription of p16(INK4), and that this effect is GC-box dependent. We also found that the transcription factor Sp1 plays a key role in this event. Upregulation of Sp1 contributes to the transcriptional activation and protein level of p16(INK4) mediated by p21(Waf1), and is a potential point of cooperation between the p16/pRb and p14 (ARF)/p53 tumor suppressor pathways.
doi_str_mv	10.1016/S0014-5793(04)00352-7
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subjects	Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis Cyclin-Dependent Kinase Inhibitor p16 - genetics Cyclin-Dependent Kinase Inhibitor p21 Cyclins - genetics Cyclins - physiology HeLa Cells Humans Mutation Promoter Regions, Genetic - genetics Sp1 Transcription Factor - genetics Sp1 Transcription Factor - physiology Transcriptional Activation Transfection Tumor Suppressor Protein p14ARF Up-Regulation
title	Sp1 is involved in the transcriptional activation of p16(INK4) by p21(Waf1) in HeLa cells
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