The effects of different anticoagulants on routine canine plasma biochemistry

The effects of heparin, ethylenediaminetetraacetic acid (EDTA), sodium citrate and sodium fluoride/potassium oxalate on plasma biochemistry results in dogs were studied and compared with serum. Blood specimens from 10 apparently clinical healthy dogs were collected and placed in different tubes cont...

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Bibliographic Details
Published in:The veterinary journal (1997) 2004-05, Vol.167 (3), p.294-301
Main Authors: Cerón, José J., Martı́nez-Subiela, Silvia, Hennemann, Carla, Tecles, Fernando
Format: Article
Language:English
Subjects:
Animals
Anticoagulants - pharmacology
Blood Chemical Analysis - methods
Blood Chemical Analysis - veterinary
Citrates - pharmacology
Dog
Dogs - blood
Edetic Acid - pharmacology
EDTA
Female
Heparin
Heparin - pharmacology
Male
Oxalates - pharmacology
Sodium citrate
Sodium fluoride
Sodium Fluoride - pharmacology
Specimen Handling - methods
Specimen Handling - veterinary
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Summary:The effects of heparin, ethylenediaminetetraacetic acid (EDTA), sodium citrate and sodium fluoride/potassium oxalate on plasma biochemistry results in dogs were studied and compared with serum. Blood specimens from 10 apparently clinical healthy dogs were collected and placed in different tubes containing each anticoagulant tested. Differences in albumin, acetylcholinesterase, ionized calcium and potassium were found between serum and heparinized plasma. Most metabolites and enzymes did not show any variation, but significant decreases in electrolytes, alkaline phosphatase, acetylcholinesterase, bile acids, fructosamine and albumin were found when EDTA was used. Sodium citrate produced a 10–15% decrease in most metabolites and enzymes, possibly due to a sample dilution effect. Sodium fluoride/potassium oxalate produced haemolysis which may have influenced changes in some biochemical parameters.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2003.09.009