Role of Histone Tails in the Conformation and Interactions of Nucleosome Core Particles

The goal of this work was to test the role of the histone tails in the emergence of attractive interactions between nucleosomes above a critical salt concentration that corresponds to the complete tail extension outside the nucleosome [Mangenot, S., et al (2002) Biophys. J. 82, 345−356; Mangenot, S....

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Bibliographic Details
Published in:Biochemistry (Easton) 2004-04, Vol.43 (16), p.4773-4780
Main Authors: Bertin, Aurélie, Leforestier, Amélie, Durand, Dominique, Livolant, Françoise
Format: Article
Language:English
Subjects:
Animals
Cattle
Histones - chemistry
Models, Chemical
Nucleosomes - chemistry
Osmolar Concentration
Peptide Fragments - chemistry
Protein Conformation
Protein Structure, Tertiary
Salts - chemistry
Scattering, Radiation
Solutions
Trypsin - chemistry
X-Rays
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Summary:The goal of this work was to test the role of the histone tails in the emergence of attractive interactions between nucleosomes above a critical salt concentration that corresponds to the complete tail extension outside the nucleosome [Mangenot, S., et al (2002) Biophys. J. 82, 345−356; Mangenot, S., et al (2002) Eur. Phys. J. E 7, 221−231]. Small angle X-ray scattering experiments were performed in parallel with intact and trypsin tail-deleted nucleosomes with 146 ± 3 bp DNA. We varied the monovalent salt concentration from 10 to 300 monovalent salt concentration and followed the evolution of (i) the second virial coefficient that characterizes the interactions between particles and (ii) the conformation of the particle. The attractive interactions do not emerge in the absence of the tails, which validates the proposed hypothesis.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi036210g