Transcellular transport of 4-iodo-L-meta-tyrosine via system L across monolayers of kidney epithelial cell line LLC-PK1

The substance 4-[(125)I]iodo-L-meta-tyrosin (4-[(125)I]mTyr) is a radioiodinated amino acid that exhibits high in vivo stability and rapid renal elimination in vivo. We investigated transport of 4-[(125)I]mTyr in LLC-PK(1) (porcine kidney epithelial cell line) monolayers grown on collagen-coated, mi...

Full description

Saved in:
Bibliographic Details
Published in:Nuclear medicine and biology 2004-05, Vol.31 (4), p.477-482
Main Authors: Shikano, Naoto, Kawai, Keiichi, Nakajima, Syuichi, Kubodera, Akiko, Kubota, Nobuo, Ishikawa, Nobuyoshi, Saji, Hideo
Format: Article
Language:English
Subjects:
Amino Acid Transport System L - metabolism
Animals
Biological Transport, Active - physiology
Cell Division - physiology
Cell Line
Cell Membrane - metabolism
Epithelial Cells - cytology
Epithelial Cells - metabolism
Iodine Radioisotopes - pharmacokinetics
Kidney - cytology
Kidney - growth & development
Kidney - metabolism
Radiopharmaceuticals - pharmacokinetics
Swine
Tyrosine - pharmacokinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The substance 4-[(125)I]iodo-L-meta-tyrosin (4-[(125)I]mTyr) is a radioiodinated amino acid that exhibits high in vivo stability and rapid renal elimination in vivo. We investigated transport of 4-[(125)I]mTyr in LLC-PK(1) (porcine kidney epithelial cell line) monolayers grown on collagen-coated, micro-porous membrane filters. We found that 4-[(125)I]mTyr transport in LLC-PK(1) cells was carrier-mediated and sodium-independent, and that 4-[(125)I]mTyr transport was similar to that of L-Tyr and 3-iodo-alpha-methyl-L-tyrosine. The results of the inhibition experiments suggest that 4-[(125)I]mTyr transport is predominantly mediated by a L-type amino acid transporter 1-like porcine homologue (a component of system L) in both basolateral and apical membrane.
ISSN:0969-8051
DOI:10.1016/j.nucmedbio.2003.12.006