Immunohistochemical detection of fibronectin and tenascin in incised human skin injuries

• Published in: Forensic science international 2002-04, Vol.126 (2), p.118-122
• Main Authors: Ortiz-Rey, J.A, Suárez-Peñaranda, J.M, Da Silva, E.A, Muñoz, J.I, San Miguel-Fraile, P, De la Fuente-Buceta, A, Concheiro-Carro, L
• Format: Article
• Language: English
• Subjects: Abdomen; Adult; Aged; Autolysis; Biological and medical sciences; Dermis; Diagnosis; Extracellular matrix; Female; Fibronectin; Fibronectins - metabolism; Forensic medicine; Forensic Medicine - methods; Hemorrhage; Humans; Immunohistochemistry; Immunoreactivity; Incised wounds; Inflammation; Injuries; Injuries of the skin. Diseases of the skin due to physical agents; Injury prevention; Male; Medical sciences; Microscopy; Middle Aged; Monoclonal antibodies; Postmortem Changes; Public health. Hygiene; Public health. Hygiene-occupational medicine; Skin; Skin - injuries; Skin - pathology; Smooth muscle; Staining; Tenascin; Tenascin - metabolism; Traumas. Diseases due to physical agents; Vitality; Wounds; Wounds, Stab - classification; Wounds, Stab - metabolism; Wounds, Stab - pathology
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/S0379-0738(02)00032-4

Immunohistochemical detection of molecules involved in inflammatory reaction can be useful for the diagnosis of vitality in skin wounds. We studied the expression of fibronectin (FN) and tenascin (TN) in 58 human skin wounds (48 vital and 10 postmortem). The age of vital injuries ranged from 3 min to 8 h and postmortem specimens were collected after a postinfliction interval of 15–180 min. One hundred thirty-seven formalin-fixed paraffin-embedded sections (mean: 2.3 sections per case) were stained with each of two monoclonal antibodies against FN and TN using the streptABC technique. A reticular staining for FN in wound edge and dermis was observed in 50% of vital specimens versus 0% in postmortem cases. Immunoreactivity was reduced in 10 autolysed cases. FN positivity exclusively at the injury margin was observed in 39.4% of vital wounds and 10% of postmortem cases. TN was negative in all specimens. Vital and postmortem hemorrhage areas showed positivity for FN and TN. Due to its low sensitivity, immunohistochemical analysis of FN is useful for determining vitality only in a minority of cases. Different factors in everyday practice, including autolysis and technical problems often produce false negative reactions with the result that FN cannot be regarded as a reliable parameter of vitality. Positive reactions (network staining) are more valuable than negativity but are not pathognomonic. Both vital and postmortem hemorrhages show an enhanced positivity for FN and TN, thus impeding the diagnosis.