Enzymatic Manipulation of DNA−Nanomaterial Constructs

• Published in: Journal of the American Chemical Society 2002-07, Vol.124 (26), p.7644-7645
• Main Authors: Yun, C. Steven, Khitrov, Gregory A, Vergona, Danielle E, Reich, Norbert O, Strouse, Geoffrey F
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Deoxyribonucleases, Type II Site-Specific - chemistry; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA - chemistry; DNA - metabolism; DNA Adducts - chemistry; Dna, deoxyribonucleoproteins; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Nanotechnology - methods; Nucleic acids; Particle Size; Site-Specific DNA-Methyltransferase (Adenine-Specific) - chemistry; Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja025971o

The demonstration and control of biofunction between inorganic nanomaterials and biological scaffolding is crucial to the development of the field of biomaterials. Although unique hierarchical structures can be generated, the impact of nanosized materials on the biological activity of DNA−protein interactions is relatively unknow. Using highly selective proteins that induce sequence-specific conformational perturbations within DNA, we demonstrate the absolute maintenance of biofunction for biomaterials composed of duplex DNA appended with 1.4-nm Au particles. Enzyme activity and DNA binding affinities (K d) are unaltered by the nanoparticle−DNA conjugates. Our results provide a foundation for interfacing more complex and diverse protein−DNA-systems.