Control of Mouse hils1 Gene Expression During Spermatogenesis: Identification of Regulatory Element by Transgenic Mouse

Histone H1-like protein in spermatids 1 (Hils1) is a testis- specific histone H1-like protein exclusively expressed in haploid spermatids and should be involved in chromatin remodeling during mouse spermatogenesis. Spatial and temporal regulation of the hils1 gene expression would be critical for th...

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Bibliographic Details
Published in:Biology of reproduction 2004-05, Vol.70 (5), p.1239-1245
Main Authors: Iguchi, Naoko, Tanaka, Hiromitsu, Yamada, Shuichi, Nishimura, Hiromi, Nishimune, Yoshitake
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Contents
DNA-Binding Proteins - genetics
Fluorescence
Fundamental and applied biological sciences. Psychology
gamete biology
Gene Expression Regulation
gene regulation
Genome
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Green Fluorescent Proteins - physiology
Indicators and Reagents - metabolism
Male
Mammalian male genital system
Mice
Mice, Transgenic
Molecular Sequence Data
Morphology. Physiology
Promoter Regions, Genetic
Protein Processing, Post-Translational
Regulatory Sequences, Nucleic Acid
RNA, Messenger - metabolism
spermatid
spermatogenesis
Spermatogenesis - physiology
testis
Testis - metabolism
Transcription, Genetic
Vertebrates: reproduction
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Summary:Histone H1-like protein in spermatids 1 (Hils1) is a testis- specific histone H1-like protein exclusively expressed in haploid spermatids and should be involved in chromatin remodeling during mouse spermatogenesis. Spatial and temporal regulation of the hils1 gene expression would be critical for the formation of functional sperm, controlled at both transcriptional and translational levels. Previously, we reported that transcripts of the hils1 gene are exclusively expressed in mouse testis from 23 days of age whereas the Hils1 protein is not detected until 28 days of age, suggesting that hils1 is a member of a class of translationally regulated genes. By analyzing transgenic mice, we could demonstrate that 318-base pair (bp) 5′-proximal region corresponding to the first 70-bp proximal TATA-less promoter, and 248 bp of 5′-untranslated region is sufficient to confer testis- and spermatid-specific transcription as well as posttranscriptional control of the mouse hils1 gene in vivo.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.103.024760