moss Physcomitrella patens releases a tetracyclic diterpene

The presence of the tetracyclic diterpene 16alpha-hydroxykaurane (16alpha-hydroxy-ent-kaurane, C20H34O, CAS 5524-17-4) was detected in sterile cell cultures of the moss Physcomitrella patens (Hedw.) B.S.G. using gas chromatography and mass spectrometry. 16alpha-hydroxykaurane was found to be a major...

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Bibliographic Details
Published in:Plant cell reports 2004-05, Vol.22 (10), p.780-786
Main Authors: Schwartzenberg, K. von, Schultze, W, Kassner, H
Format: Article
Language:English
Subjects:
Bryopsida - chemistry
crystal structure
Crystals
Culture Media
Diterpenes, Kaurane - chemistry
Diterpenes, Kaurane - metabolism
diterpenoids
Gas chromatography
Gas Chromatography-Mass Spectrometry
hydroxykaurane
Magnetic Resonance Spectroscopy
Mass spectrometry
Mosses
mosses and liverworts
nuclear magnetic resonance spectroscopy
Physcomitrella patens
spectral analysis
tetracyclic diterpene
Time Factors
volatile organic compounds
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Summary:The presence of the tetracyclic diterpene 16alpha-hydroxykaurane (16alpha-hydroxy-ent-kaurane, C20H34O, CAS 5524-17-4) was detected in sterile cell cultures of the moss Physcomitrella patens (Hedw.) B.S.G. using gas chromatography and mass spectrometry. 16alpha-hydroxykaurane was found to be a major lipid compound in P. patens, with an estimated intracellular concentration of up to 0.84 mmol/l and an extracellular concentration of up to 9.3 micromol/l. The overall content of 16alpha-hydroxykaurane (in milligrams) produced per culture reached 0.37-fold that of chlorophyll a+b. In agar cultures with low air exchange, 16alpha-hydroxykaurane forms needle-like crystals on tissue and on the inner surface of the culture vessels, indicating that it is being released into the atmosphere. Solid phase microextraction confirmed the air-bound release of 16alpha-hydroxykaurane. To our knowledge this is the first report on the release of a plant-derived tetracyclic diterpene into the air.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0754-6