HLA-DQB1, -DQA1, -DRB1 linkage disequilibrium and haplotype diversity in a Mestizo population from Guadalajara, Mexico

• Published in: Tissue antigens 2004-05, Vol.63 (5), p.458-465
• Main Authors: Cortes, L.M., Baltazar, L.M., Perea, F.J., Gallegos-Arreola, M.P., Flores, S.E., Sandoval, L., Olivares, N., Lorenz, M.G.O., Xu, H., Barton, S.A., Chakraborty, R., Rivas, F.
• Format: Article
• Language: English
• Subjects: European Continental Ancestry Group; haplotype; Haplotypes; HLA class II; HLA-DQ alpha-Chains; HLA-DQ Antigens - genetics; HLA-DQ beta-Chains; HLA-DQA1; HLA-DQB1; HLA-DR Antigens - genetics; HLA-DRB1; HLA-DRB1 Chains; Humans; Indians, North American; Linkage Disequilibrium; Mexico; Polymerase Chain Reaction
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1111/j.0001-2815.2004.00202.x

:  HLA‐DQB1, ‐DQA1, and ‐DRB1 genes were typed by polymerase chain reaction with sequence‐specific primer (PCR‐SSP) in 159 healthy volunteers from 32 families living in Guadalajara, Mexico. Three‐locus genotype data from all family members were used to infer haplotypes in 54 unrelated individuals of the sample, from which estimate of segregating haplotype frequencies and linkage disequilibrium (LD) between loci were computed. Genotype distributions were concordant with Hardy–Weinberg expectations (HWE) for all three loci, and allele distributions were similar to the ones observed in other Latin‐American populations. Of the 56 distinct three‐site (DQB1‐DQA1‐DRB1) haplotypes observed in the sample, the five most common (i.e., with frequencies of five counts or more) were: *0302‐*0301‐*04, *0201‐*0201‐*07, *0301‐*0501‐*14, *0402‐*0401‐*08, and *0501‐*0101‐*01. These common three‐locus haplotypes also contributed to the majority of the significant two‐locus linkage disequilibria of these three sites.